Magnetic separation cell sorting

Porter,)., Robinson,)., Pickup, R. and Edwards, C.). (1998) An evaluation of lectin-mediated magnetic bead cell sorting for the targeted separation of enteric bacteria. Journal of Applied Microbiology, 84, ITl- il. 

Magnetic cell sorting, 26 971-972 Magnetic concentration and purification, 15 442-457. See also Magnetic separators... 

Magnetic (iron oxide core) microspheres have been imprinted with S-pro-pranolol. The magnetism allowed the facile separation of the imprinted beads from the liquid matrix. The particles exhibited the expected affinity towards the template molecule. This technique was also proposed as a putative tool for cell sorting [81]. 

Kantor, A. B., Gibbons, I., Miltenyi, S., and Schmitz, J. (1998) Magnetic cell sorting with colloidal superparamagnetic particles, in Cell Separation Methods and Applications (Recktenwald, D., and Radbruch, A., eds.), Marcel Dekker, Inc., New York, pp. 153. 

Owen CS (1982) Magnetic cell sorting. In Cell Separation Methods and Applications, Vol. 2, TG Pretlow and TP Pretlow, Eds. Academic Press, Orlando, FL. 

In magnetic cell sorting (MACS), cells labeled with primary antibody are incubated with a ferrous conjugated second antibody. Labeled cells are separated by decanting the cell suspension in a magnetic field. Pizzonia et al. [101] and Bacskai and co-authors [102] introduced MACS for immunoselection of murine distal tubule cells. Baer et al. [103] used MACS for im-munodissection of human proximal and distal tubule... 

Stanciu LA, Shute J, Holgate ST, Djukanovic R. Production of IL-8 and IL-4 by positively and negatively selected CD4-I- and CD8-1- human T cells following a four-step cell separation method including magnetic cell sorting (MACS). J Immunol Methods 1996 189 107-115. 

The batteries are mechanically and magnetically sorted into different fractions according to their composition, i.e. after hand sorting, during which incorrectly sorted batteries and larger batteries are removed, they are sorted by size, and the R9 cells are sieved out. The round cells are run across a magnetic separator. The non-... 

Hematopoietic cells can be identified by cell surface markers via flow cytometry. If testing of a purified population of either HSCs or differentiated cells is desired, hematopoietic cells can be isolated by fluorescence activated cell sorting (FACS) or magnetic separation by either positive or negative selection of known hematopoietic surface markers. Continued study of the markers which distinguish HSCs and their derivatives to clearly identify HSCs must be done in order to further examine and utilize these various cell populations. 

Lab-on-a-Chip devices for particle and ceU separation are used to either remove particles of interest, sort a mixed population of particles into subpopulations of like particles, or concentrate (enrich) particles for downstream processing within microdevices. Methods for particle or cell separations exploit specific physical properties which include fluorescence-based, magnetic-based, affinity-based, and cell density gradient-based separations depending on the properties of the particles of interest. Particle separation devices are often geared toward biomedical research and diagnostics particularly in blood cell separation and blood diagnostics. 

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