Lymphocytes subset analysis

Lymphocyte subset analysis flow cytometry of PMBC and cells of lymphoid organs (spleen, lymph nodes)... 

For classification in routine lymphocyte subset analysis, expert analysis is often fully adequate. For polychromic flow cytometry, particularly when both lineage and functional phenotypes are being evaluated, more sophisticated analysis may be required. Indeed, more sophisticated analysis can define subpopulations that cannot be discriminated by any combination of two-dimensional projections of multicolor data (Zamir et al., 2005). One of the first steps in enabling more sophisticated downstream analysis is ensuring acquisition of robust data. Some of the recently described analytical methods for high-dimensional flow cytometry data are listed in Table 4.2-1. 

Romasko, F., Rosenburg, J., and Wybran, J. (1985) An immunogold silver method for the light microscopic analysis of blood lymphocyte subsets with monoclonal antibodies Am. J. Clin Pathol. 84, 307-316. 

Kasaian MT, Ikematsu H and Casali P, Identification and analysis of a novel human surface CD5-B lymphocyte subset producing natural antibodies, J. Immunol., 148 2690-2702, 1992. 

Figure 18.4 Postnatal distribution of lymphocyte subsets by immunophentyping analysis in immature cynomolgus monkeys. Typically CD4-CD8 ratio declines during postnatal development, (e.g., from 2.2 to 1.6 within 12 months in this data set). Others reported a ratio decrease of 3.1 to 1.5 within 12 months for the same species [54]. Data represent mean values + SEM of 26 to 32 animals (males and females combined). CD3+, O CD4+, A CD20 T CD8+, CD16+.

Other flow cytometry and immunhistochemical analysis of lymphoid tissues for peripheral lymphocyte subsets humoral immunity... 

Booss, J., Esiri, M. M., Tourtellotte, W. W., and Mason, D. Y., Immunohistological analysis of T lymphocyte subsets in the central nervous system in chronic progressive multiple sclerosis, J. Neurol. Sci., 62, 219, 1983. 

Sreenan JJ, Tbakhi A, Edinger MG, Tubbs RR. The use of isotypic control antibodies in the analysis of CD3+ and CD3+, CD4+ lymphocyte subsets by flow cytometry. Are they really necessary Arch Pathol Lab Med 1997 121 118-121. 

Flow Cytometry Analysis. Flow cytometry analysis of blood and tissues has the advantage of allowing enumeration of lymphocyte subsets (see Chapters... 

The percentage of different WBC in peripheral blood is shown in Table 3. Since the lymphocyte component is approximately 20-40% of the nucleated cell count, FACS analysis of lymphocyte subsets is done with approximately 10000 cells yielding an appropriate number of lymphocytes to analyse. In contrast, since the number of CD34 cells is often less that 1% of WBC in blood. BM, and PBSC harvests, quantitation requires at least 20000 ceU to be analysed. 

Analysis of lymphocyte subsets in peripheral blood and bone marrow harvests from unrelated donors... 

Analysis of lymphocyte subsets after transplantation or autologous rescue... 

Only a few representative applications have been cited above. In addition it may be said that flow cytometry finds numerous applications in the field of cell differentiation, immunology, parasitology, sperm analysis, food science, pharmacology and toxicology, cancer oiology and carcinogenesis, bone marrow analysis, tissue typing and lymphocyte applications, T-cell subset analysis. 

Analysis of lymphocyte subsets after transplantation or autologous rescue 384 Measurement of intracellular C5doldnes in mononuclear cells (MNC) and T cells after transplantation or autologous rescue 384... 

Costabel U, Bross KJ, Reuter C, Ruhle KH, Matthys H Alterations in irrrmrmoregulatory T-cell subsets in cigarette smokers. A phenotypic analysis of bronchoalveolar and blood lymphocytes. Chest 1986 90 39 4 erratum 1986 92 1124. 

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