LUSH protein

OBP) to elicit an olfactory response. The LUSH protein was found to cocrystallize with small alcohols and to bind a variety of ligands (Table 9.1). A similar picture emerges from electrophysiological recordings of receptor cells from the giant silkmoth Antheraea polyphemus, in which direct perfusion of the sensory hair with various PBP/odorant complexes elicits different response patterns. The... 

Using a specific antibody, Shanbhag and collaborators [34] demonstrated that LUSH is expressed in sensilla trichodea of the Drosophila antennae along with two other putative odorant-binding proteins Obp83a (PBPRP-3, OS-F) and Obp83b (OS-E). When antennal sections of the LUSH-deficient mutant were la-... 

Fig. 9 Alignment of the amino acid sequences of pheromone-binding proteins from the silkworm moth B. mori and the cockroach L. maderae, BmorPBP and LmadPBP,respectively and a putative odorant-binding protein from D. melanogaster, LUSH. In LmadPBP and LUSH the N-terminal sequence of the mature proteins were predicted by cleaving signal peptides in silico [28,79], whereas in BmorPBP this was confirmed by the sequence of the isolated protein [38]...

Lush RM, Dsenderowicz A, Figg WD, Headlee D, Inoue K, Sausville EA (1997) Surprising pharmacokinetics of UCN-01 in patients with refractory neoplasms may be due to high degree of plasma protein binding. Proc Am Assoc Cancer Res 38 600... 

Kim M. S., Repp A. and Smith D. P. (1998) LUSH odorant-binding protein mediates chemosensory responses to alcohols in Drosophila melanogaster. Genetics 150, 711-721. 

Relationships of moth and D. melanogaster OBPs This hypothesis may imply the existence of OS-E and LUSH orthologs in some moth species. A protein similar to LUSH has been identified from the antennae of A. ipsilon (Picimbon, unpublished data). In addition, the PBPRPs from D. melanogaster display significant similarities to a specific subclass of moth OBPs that includes binding proteins whose function is unknown, the so-called antennal binding proteins-X (ABPX). 

Lush, I. E. Genetic polymorphisms in the egg albumin proteins of the domestic fowl. Nature 189, 981 (1961). 

Boudoulas S, Lush RM, McCall NA et al. (1996) Plasma protein binding of phenylacetate and phenylbutyrate, two novel antineoplastic agents. TherDrug Monit 18 714 Christensen JH, Andreasen F, Jensen EB (1980) The binding of thiopental to serum proteins determined by ultrafiltration and equilibrium dialysis. Acta Pharmacol Toxicol 47 24-32... 

The UniProt entry for human NTE lists three variants attributed to alternative splicing (UNIPROT, 2008). Although UniProt has adopted isoform-1 as the canonical sequence, this chapter defines human NTE as isoform-2, which is the sequence initially reported by Lush et al. (1998), consisting of 1,327 amino acids and having a molecular weight without posttranslational modifications of 146 kDa. The protein has a transmembrane domain near the N-terminus (residues 9-31) and three putative cyclic nucleotide binding domains (residues 163-262, 480-573, and 597-689), in addition to the patatin domain (residues 933-1,099), which contains the active site (Wijeyesakere et al., 2007). 

Lush, M. J., Li, Y., Read, D.J., Willis, A.C., Glynn, P. (1998). Neuropathy target esterase and a homologous Drosophila neurodegeneration-associated mutant protein contain a novel domain conserved from bacteria to man. Biochem. J. 332 1-4. 

Wistrand M et al (2006) A general model of G protein-coupled receptor sequences and its application to detect remote homologs. Protein Sci 15 509-521 Wong ST et al (2000) Disruption of the type III adenylyl cyclase gene leads to peripheral and behavioral anosmia in transgenic mice. Neuron 27 487-497 Xu P et al (2005) Drosophila OBP LUSH is required for activity of pheromone-sensitive neurons. Neuron 45 193-200... 

Fig. 9.2 (continued). D) AmelASPl complexed with n-butyl-benzene-sulfonamide (a plastics additive),E) LmaPBP with H3B2 bound,F) LUSH complexed with ethanol. The top row of structures reveals the overall fold of the protein, while the bottom row is a cutaway view of the molecule with the protein surface and binding eavity shown as mesh. Images were prepared using PyMOL (Delano, W.L. 2004). 

KIM, M.-S., REPP, A., SMITH, D.P., Lush odorant-binding protein mediates... 

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