Proteins antennal binding

Relationships of moth and D. melanogaster OBPs This hypothesis may imply the existence of OS-E and LUSH orthologs in some moth species. A protein similar to LUSH has been identified from the antennae of A. ipsilon (Picimbon, unpublished data). In addition, the PBPRPs from D. melanogaster display significant similarities to a specific subclass of moth OBPs that includes binding proteins whose function is unknown, the so-called antennal binding proteins-X (ABPX). 

Using a specific antibody, Shanbhag and collaborators [34] demonstrated that LUSH is expressed in sensilla trichodea of the Drosophila antennae along with two other putative odorant-binding proteins Obp83a (PBPRP-3, OS-F) and Obp83b (OS-E). When antennal sections of the LUSH-deficient mutant were la-... 

Vogt R. G., Koehne A. C., Dubnau J. T. and Prestwich G. D. (1989) Expression of pheromone binding proteins during antennal development in the gypsy moth Lymantria... 

Only a few proteins from antennal extracts are were able to bind the four compounds tested (Figure 17.3). Fraction 29 included proteins with sequences DAPAA and SEEDK no proteins in this fraction bound compounds. A protein with sequence AKLTT from fractions 30, 31 and 32 bound compounds. The full sequence of the AKLTT protein has been obtained by molecular cloning and identified as a CSP (Jacquin-Joly et al., unpublished, accession number AY026760 see section 17.7). The sequence AKLTT is also found in band 1 of fraction 33 (Figure 17.2), but here this protein fails to bind any compound (Figure 17.3). 

Figure 17.3 Binding experiments between tritiated compounds and RP-HPLC-purified antennal proteins. Each fraction corresponds to an aliquot of the purification. One pCi of each pheromonal compound was incubated with 20 pL of each fraction (sample buffer). The films were exposed to the...

Our binding assays also identified M. brassicae proteins of the OBP-Type 2 class. Several of these proteins from both male and female antennal extracts were observed to bind the four tritium-labeled analogs of Zll-16 Ac, 16 Ac,... 

The binding of pheromone molecules by antennal proteins has been observed (340, 341) and transport of a pheromone molecule is considered by some as an integral part of the overall sequence leading to perception (334, 342). In addition, antennae of males and females of Trichoplusia ni contain an enzyme capable of hydrolyzing its pheromone, (2)-7-dodecenyl... 

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