Electrophysiological recording

Studies on the biochemistry of the taste system should take into account results obtained at other levels, such as electrophysiological recordings and, particularly, behavioral responses to taste stimuli. The term sweetness should strictly be used only in studies conducted on humans, because the description of taste modality is a verbal response. It is usually concluded that positive behavioral responses in animals, that is, preferences, or electrophysiological response to a stimulus compound that is known to be sweet to man, are due to the sweet taste. This may not necessarily be true in some cases, because behavioral or electrophysiological response may result from other taste modalities. It is, therefore, critical that comparative aspects be carefully interpreted. 

This section reviews four different experimental approaches that together argue in favor of a temporal analysis function of lobster olfaction. The experiments include high-resolution measurements of turbulent odor dispersal and lobster sampling behavior, electrophysiological recording of in situ single cell responses to controlled and chaotic stimuli, and behavioral analysis of orientation and localization of odor sources. 

Stiihmer W, Parekh AB. 1995. Electrophysiological recordings from Xenopus oocytes. Single-channel recording. Second edition. Sakmann B, Neher E, editors. New York Plenum Press pp 341-356. 

A number of chemo- and mechanoreceptors participate in the male behaviors. The female contact pheromone is detected by chemosensilla on the antennae and labial and maxillary palps (Ramaswamy and Gupta, 1981). The number of these sensilla increases dramatically during the metamorphic molt, and much more so in males than in females. Unfortunately, no electrophysiological recordings have been conducted, and the specific sensillum type that responds to the contact pheromone... 

As Ca2+ is important for endocytosis, the sustained secretory activity in its absence eventually depletes NMJ terminals (Longenecker et al. 1970 Ceccarelli and Hurlbut 1980), but not synaptosomes (Watanabe and Meldolesi 1983), of all vesicles. Electrophysiological recordings at NMJs show that in the absence of Ca2+a-LTX causes a large but slow rise in the frequency of spontaneous exocy-totic events, which then slow down and cease altogether bursts of miniatures are never observed. 

Derby, C. D., Single unit electrophysiological recordings from crustacean chemoreceptor neurons, in Experimental Biology of Taste and Olfaction Current Techniques and Protocols, Spielman, A. I. and Brand, J. G., Eds., CRC Press, Boca Raton, FL, 1995, 241. 

Fig. 7. Effect of treatment with finasteride or clomiphene on expression of the 6 and 72 subunits expression and GABAergic tonic currents during pregnancy. Finasteride (25 mg/kg), clomiphene (5 mg/kg), or vehicle was administered daily from day 12 to day 18 of pregnancy, and rats were analyzed at day 19 of pregnancy for both immunohistochemistry (A—D), and electrophysiological recordings (E—H). In graphs A—D, data are expressed as percentage change relative to the...

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