Lung epithelial cells

Lemercier, J.-N. Meier, B. Gomez, J. D. Thompson, J. A. Inhibition of glutathione S-transferase Pl-1 in mouse lung epithelial cells by the tumor promoted 2,6,di-tert-butyl-4-methylene-2,5-cylcohexadienone (BHT-quinone methide) protein adducts investigated by electrospray mass spectrometry. Chem. Res. Toxicol. 2004, 17, 1675-1683. 

FIGURE 10.3 Structure of the BHTOH-KLH conjugate utilized for raising polyclonal antibodies. Western blots from 2D gels of cytosolic proteins isolated from mouse lung epithelial cells. The blot on the right is from cells incubated with BHT-QM and the blot on the left is from untreated cells. Immunoreactive proteins were identified by electrospray LC-MS analysis of the tryptic digests. Source From Ref. 39, with permission from the American Chemical Society. 

Sun, Y. Dwyer-Nield, L. D. Malkinson, A. M. Zhang, Y. L. Thompson,J. A. Responses of tumorigenic and non-tumorigenic mouse lung epithelial cell lines to electrophilic metabolites ofthe tumor promoter butylated hydroxytoluene. Chem.-Biol. Interact. 2003, 145, 41-51. 

Hmbach, L.K., Wick, P., Manser, P., Grass, R.N., Bruinink, A., and Stark, W.J. (2007) Exposure of engineered nanopartides to human lung epithelial cells influence of chemical composition and catalytic activity on oxidative stress. Environmental Science and Technology,... 

Even if in vitro models can mimic only partially the complex responses in vivo, the behavior of lung epithelial cells in in vitro experiments resembles in some respects the pathological observations of in vivo experiments. First, the formation of... 

Herzog, E. et al. (2009) Dispersion medium modulates oxidative stress response of human lung epithelial cells upon exposure to carbon nanomaterial samples. Toxicology and Applied Pharmacology, 236 (3), 276-281. 

Dermal toxicity due to JP-8 may also be attributed to increased free radical formation that may be involved in the development of skin sensitization [45], Following dermal exposure to JP-8, increasing levels of oxidative species are observed [46], In vitro studies with rat lung epithelial cells demonstrated that JP-8-induced cell death is inhibited by exogenous glutathione or the thiol-containing antioxidant N-acetyl-cysteine... 

Boulares, A.H., et al., Roles of oxidative stress and glutathione depletion in JP-8 jet fuel-induced apoptosis in rat lung epithelial cells, Toxicol. Appl. Pharmacol., 180, 92, 2002. 

Singh, J., Pritchard, D. E., Carlisle, D. L., McClean, J. A., Montaser, A., Orenstein, J. M. and Patierno, S. R. (1999). Internalization of carcinogenic lead chromate particles by cultured normal human lung epithelial cells formation of intracellular lead-inclusion bodies and induction of apoptosis, Toxicol. Appl. Pharmacol., 161, 240-248. 

Fiegel J, Ehrhardt C, Schaefer UF, Lehr CM, Hanes J (2003) Large porous particle impingement on lung epithelial cell monolayers—toward improved particle characterization in the lung. Pharm Res 20(5) 788-796. 

Woollhead AM, Baines DL (2006) Forskolin-induced cell shrinkage and apical translocation of functional enhanced green fluorescent protein-human alphaENaC in H441 lung epithelial cell monolayers. J Biol Chem 281 (8) 5158—5168... 

Abraham, G., C. Kneuer, C. Ehrhardt, W. Honscha, and F. R. Ungemach. 2004. Expression of functional beta2-adrenergic receptors in the lung epithelial cell lines 16HBE14o-, Calu-3 and A549. Biochim Biophys Acta 1691(2-3) 169-79. 

Blaine, S. A., M. Wick, C. Dessev, and R. A. Nemenoff. 2001. Induction of cPLA2 in lung epithelial cells and non-small cell lung cancer is mediated by Spl and c-Jun. J Biol Chem 276(46) 42737 13. 

Bose S, Basu M, Banerjee AK (2004) Role of nucleolin in human parainfluenza virus type 3 infection of human lung epithelial cells. J Virol 78 8146-8158 Bouche G, Caizergues-Ferrer M, Bugler B, Amalric F (1984) Interrelations between the maturation of a 100 kDa nucleolar protein and pre rRNA synthesis in CHO cells. Nucleic Acids Res 12 3025-3035 Bouche G, Gas N, Prats H, Baldin V, Tauber JP, Teissie J, Amalric F (1987) Basic fibroblast growth factor enters the nucleolus and stimulates the transcription of ribosomal genes in ABAE cells undergoing GO-Gl transition. Proc Natl Acad Sci U S A 84(19) 6770-6774. 

Morris CM, Smith GJ (1992) Altered levels and protein kinase C-mediated phosphorylation of substrates in normal and transformed mouse lung epithelial cells. Exp CeU Res 200 149-155... 

G. R. Borthry, W. E. Antholine, J. M. Myers, and C. R. Myers, Reductive activation of hexavalent chromium by human lung epithelial cells Generation of Cr(V) and Cr(V)-thiol species, J. Inorg. Biochem., 102 (2008) 1449-1462. 

Cytotoxic effect. Gas phase of mainstream cigarette smoke, in monolayer culture of mouse lung epithelial cells, produced an increase in cytotoxicity in a dose-dependent manner. Cell viability of cultures exposed to gas phase with only the nonorganic components was equivalent to controls. Removal of volatile organic constituents resulted in almost elimination of cytotoxicity of the smoke . Smoke condensate and tobacco extract, at high concentrations in Lewis lung adenocarcinoma cells and mice spleen lymphocytes, were cytotoxic. Smaller doses increased thymidine incorporation in both cell types. Lymphocytes were more susceptible to the toxic effect of tobacco prod-... 

Piperi, A. Stavridou, M. C. Psalli-dopoulos, and J. C. Stavrides. The cytotoxic effect of volatile organic compounds of the gas phase of cigarette smoke on lung epithelial cells. Free Radic Biol Med 2003 34(3) 345-355. 

Ferrari, S., , E., Pettenazzo, A., et al. (1994). ExGen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro and in vivo. Gene Then, 4,1100-1106. 

Lichtenberg, G, Nowak, C., Gleier, K., Meckert, C.. Richter-Reichhelm, H.-B. (1995) Anchorage independent colony growth of fetal hamster lung epithelial cells after treatment with diepoxybutane. Toxicol. Lett., 75, 193-199... 

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