Liquid chromatography/mass spectrometry sample preparation

The need to understand the fate of pesticides in the environment has necessitated the development of analytical methods for the determination of residues in environmental media. Adoption of methods utilizing instrumentation such as gas chro-matography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS), liquid chromatography/tandem mass spectrometry (LC/MS/MS), or enzyme-linked immunosorbent assay (ELISA) has allowed the detection of minute amounts of pesticides and their degradation products in environmental samples. Sample preparation techniques such as solid-phase extraction (SPE), accelerated solvent extraction (ASE), or solid-phase microextraction (SPME) have also been important in the development of more reliable and sensitive analytical methods. [Pg.605]

Lant M.S. and Oxford J., 1987. Automated sample preparation online with thermospray high-performance liquid chromatography-mass spectrometry for the determination of drugs in plasma. J Chromatogr A 394 223. [Pg.296]

Ynddal, L., and Hansen, S. H. (2003). On-line turbulent-flow chromatography-high-performance liquid chromatography-mass spectrometry for fast sample preparation and quantitation.. /. Chromatogr. A. 1020 59-67. [Pg.273]

Simpson, H. Berthemy, A. Buhrman, D. Burton, R. Newton, J. Kealy, M. Wells, D. Wu, D. 1998. High throughput liquid chromatography/mass spectrometry bioanalysis using 96-well disk solid phase extraction plate for the sample preparation. Rapid Commun. Mass Spectrom., 12,75-82. [Pg.227]

Mirgorodskaya, E., Braeuer, C., Fucini, P, Lehrach, FI. and Gobom, J. (2005a) Nanoflow liquid chromatography coupled to matrix-assisted laser desorption/ionization mass spectrometry Sample preparation, data anlysis, and application to the analysis of complex peptide mixtures. Proteomics 5, 399-408. [Pg.378]

The analysis of CAD and alkamides has been completed using a variety of analytical techniques that include high-performance liquid chromatography (HPLC), capillary electrophoresis, gas chromatography (GC), GC-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR). Sample preparation methodologies utilize hexane, methanol and ethanol as the primary extraction solvents. [Pg.247]

Vuckovic D (2012) Current trends and challenges in sample preparation for global metabolomics using liquid chromatography-mass spectrometry. Anal Bioanal Chem 403(6)4523-1548... [Pg.429]

Ynddal, L. Hansen, S.H. On-Line Turbulent-Flow Chromatography-High-Performance Liquid Chromatography-Mass Spectrometry for Fast Sample Preparation and Quantitation, J. Chromatogr. A 1020(1), 59-67 (2003). [Pg.187]

Cross-validation is conducted when two validated bioanalytical methods are used within the same study or submission, for example, ELISA assay to Biacore and ELISA to a liquid chromatography/mass spectrometry. It is recommended that test samples (spiked and/or pooled incurred samples) be used to cross-validate the bioanalytical methods. Data should be evaluated using an appropriate predefined acceptance criteria or statistical method [12,13]. It should be cautioned that many times the methods that are being cross-validated may not have the same range of quantification. In these situations, it is necessary to prepare spiked samples within the range that are common to both methods for comparison. [Pg.582]

Techniques to Facilitate the Performance of Mass Spectrometry Sample Preparation, Liquid Chromatography, and Non-Mass-Spectrometric Detection... [Pg.353]

Sircar D, Subbaiah PV. Isoprostane measurement in plasma and urine by liquid chromatography—mass spectrometry with one-step sample preparation. Clin Chem 2007 53 251-258. [Pg.681]