Liquid chromatography/mass growth

RD Voyksner, T Cairns. Application of liquid chromatography-mass spectrometry to the determination of pesticides. In J Sherma, ed. Analytical methods for pesticides and plant growth regulators. New York Academic Press, 1989, pp 119-166. 

Both variolin B and its 5-deoxy derivative 19 (Scheme 6.1 Part 2) have shown promise in pharmacokinetic and in vivo studies. These compounds have been shown to have long terminal half-lives and low normal cell toxicity, however the 5-deoxy derivative demonstrated better Cmax, plasma clearance and terminal plasma half-life.318,319 Both are effective against human lung carcinoma cell lines in nude mice.104 The deoxy-variolin B showed growth inhibitory activity against human leukemic cell lines.105 A standardised method for liquid chromatography-mass spectrometry (LC-MS)/MS analysis of plasma has been developed to monitor the results of the in vivo studies.320... 

Wu, S., Jardine, I.,Hancock, W., and Karger, B. (2004) A new and sensitive on-line liquid chromatography/mass spectrometric approach for top-down protein analysis The comprehensive analysis of human growth hormone in an E. coU lysate using a hybrid linear ion trap/Fourier transform ion cyclotron resonance mass spectrometer. Rapid Communications in Mass Spectrometry, 18,2201-2207. 

Chang, J. P Kiehl, D. E. Kennington, A. 1997. Separation and characterization of the tryptic peptide mapping of recombinant bovine growth hormone by reversed-phase high-performance liquid chromatography electrospray mass spectrometry. Rapid Commun. Mass Spectrom., 11, 1266-1270. 

Liquid chromatography thermospray—mass spectrometry has been utilized to determine the identity and purity of taxol, solvent-induced degradation of cloxacillin [129], benzodiazepines, and ethanolamine-type antihistamines [130]. Electrospray LC-MS has been used for peptide mapping of the human growth hormone [131]. 

Recent advances in mass spectrometry have rendered it an attractive and versatile tool in industrial and academic research laboratories. As a part of this rapid growth, a considerable body of hterature has been devoted to the apph-cation of mass spectrometry in clinical studies. In concert with separation techniques such as hquid chromatography, mass spectrometry allows the rapid characterization and quantitative determination of a large array of molecules in complex mixtures. Herein, we present an overview of the above techniques accompanied with several examples of the use of liquid chromatography-tandem mass spectrometry in pharmacokinetics/drug metabohsm assessment during drug development. 

The use of NCW as the mobile phase in liquid chromatography was recently reviewed. In this area, in addition to its green credentials, NCW is compatible with a range of detection methods flame ionization detection, mass spectrometry (MS) and UV (to short wavelengths). The reason for the recent growth in this area is the development of more thermally stable stationary phases. It has been used to analyse a growing number of analytes (alkylbenzenes, phenols, ketones, carboxylic acids, amino acids, carbohydrates and some pharmaceuticals). For example, carbohydrates (monosaccharides, disaccharides and sugar... 

Constanzer, M. Chavez-Eng, C. Matuszewski, B. Determination of a Thermally Labile Metabolite of a Novel Growth Hormone Secretagogue in Human and Dog Plasma by Liquid Chromatography with Ion Spray Tandem Mass Spectrometric Detection, J. Chromatogr. B 760,45-53 (2001). 

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