Leukocytic Membranes

Evans, J.F., Leblanc, Y., Fitzsimmons, B.J., Charleson, S.M., Nathaniel, D. and Leveille, C. (1987). Activation ofleuko-cy te movement and displacement of [ H]-leukotriene B4 from leukocyte membrane preparations by (12 - and (12S)-hydroxyeicosate-traenoic acid. Biochem. Biophys. Acta 917, 406-410. 

In this section several recently published studies on the interaction of nonionic surfactants with a variety of biological systems, including enzymes, bacteria, erythrocytes, leukocytes, membrane proteins, low density lipoproteins and membranes controlling absorption from the gastrointestinal tract, nasal and rectal cavities, will be assessed. This is a selective account, work having been reviewed that throws light on structure-activity relationships and on mechanisms of surfactant action. 

These data along with earlier studies on a TrpE-Stel4p fusion protein show that Icmt is the sole enzymatic component necessary to methyl ester-ify the a-carboxyl of isoprenylated cysteine residues [43,49]. Unfortunately, despite significant efforts, hicmt has proven refractory to functional purification and reconstitution. Active hicmt could not be extracted with detergent from leukocyte membranes in active form nor could its enzymatic activity be restored upon reconstitution into liposomes [23]. 

Caimi, G. Eryflirocyte, datelet and polymorphonuclear leukocyte membrane dynamic properties in... 

Agranulocjdosis can be asymptomatic and, since it occurs unpredictably, regular monitoring of the leukocjde count is advisable, especially in patients concurrently receiving combination chemotherapy. Whilst the mechanism is not clear, granulocyte-agglutinating antibodies have been found, suggesting that levamisole acts as a hapten on the leukocyte membrane. 

Bazil V (1995) Physiological enzymatic cleavage of leukocyte membrane molecules. In Immunol Today 6 135-140. 

Thus, to explore the role of DHA in modulating leukocyte membrane function, we will focus on three categories of membrane proteins adhesion proteins, MHC molecules, and membrane-associated signaling proteins. In the case of adhesion proteins and signaling proteins, a few examples will be used to illustrate the point. 

Willard-Gallo, K.E., Humhlet, Y., and Symann, M., 1984, Leukocyte membrane proteins in chronic lymphocytic leukemia, as studied by two-dimensional gel electrophoresis. Clin. Chem. 30 2069-2077. 

Leukokinin the specific leukophilic which binds to the leukocyte membrane and acts as the precursor of Ihftsin (see). 

Leukophilic y-globulin predominantly yG-globu-lin and minor amounts of yA-globulin and yM-globu-lin, which coat the surface of the leukocyte membrane. See Tuftsin. 

Herzenberg, L. A. (1976) Functional studies of lymphoid cells defined and isolated by fluorescence activated cell sorter (FACS) from normal and athymic mice. 1 Leukocyte Membrane Determinants Regulating Immune Reactivity ... 

The data supported the hypothesis that the highly dynamic cell adhesion process is based on the low affinity and fast kinetics of selectin interaction. GlyCAM-1 was shown to bind multivalently to immobilized CD62L at concentrations just above its mean serum level, indicating a physiological role by interacting with CD62L embedded in leukocyte membranes. 

Parodi, A., et al., 2013. Synthetic nanoparticles functionalized with biomimetic leukocyte membranes possess cell-like functions. NaL Nanotechnol. 8 (1), 61-68. http //dx.doi. org/10.1038/nnano.2012.212. 

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