Leukocyte cultures

Biek, Y. A. E., Culvenor, C. C. J. and Jago, M. V. 1975. Comparative effects of pyrrolizidine alkaloids and related eompounds on leukocyte cultures from Potorus triadactylus. Cytobios, 14 151-160. 

Weiss, A., and Fitch, F. W. (1977). Macrophages suppress CTL generation in rat mixed leukocyte cultures. J. Immunol. 119, 510-516. 

Genetic and Neonatal Toxicity. The ability of heavy metals readily to cross the placenta and disrupt nucleic acids coupled with the high sensitivity of the fetus and neonate increases the potential dangers of congenital and neonatal toxicity. In mammalian leukocyte cultures, chromosomal aberrations have been reported with lead (112), arsenic (113), mercury (114), and methylmercury (115). Charbonneau, et al. (116) reported a lack of mutagenic effect for methylmercury. 

Effects of Major Human Brain Gangliosides on H-Thymidine Incorporation (CPMxlO- ) Into PBMC In Mixed Leukocyte Cultures... 

When these same gangliosides were added to mixed leukocyte cultures (MLC), all caused some inhibition at 12.6 nanomoles and none inhibited when 1.6 nanomoles was added (Table V). At intermediate and high concentrations, GM4 and GM3 inhibited less than GD3 and GM2. These results again indicate that their effects on the Con-A and mixed leukocyte reactions are different, and that on a molar basis different gangliosides have differential inhibitory effects. 

Elsasser-Beile, U., Willenbacher, W., Bartsch, H., Gallati, H., Monting, J., and Von Kleist, S. 1996. Cytokine production in leukocyte cultures during therapy with Echinacea extract. J. Clin. Lab. Anal. 10, 441-445. 

Hassan and Ebtekar (2002) suggested that patients with moderate clinical manifestations may be experiencing a shift from Thl to Th2 cytokine patterns since leukocyte cultures from this patient group showed a decrease in IFN-y... 

Leukocyte culture studies have demonstrated chromosomal breakage when exposed to LSD. Clastogenicity studies have also demonstrated suppression of mitosis. Other studies have shown LSD to have either no or only weakly mutagenic effects. 

Interferona (IFNa) is the major interferon produced by virus-induced leukocyte cultures the primary producer cells are null lymphocytes, and the major activities are antiviral activity and activation of NK cells. It is used in the experimental treatment of hairy cell leukemia and other selected neoplasias. 

A.S. Rosenthal, Ed., in "Immune Recognition. Proc. 9th Leukocyte Culture Conference," Academic Press, NY, 1975. 

Interferon-alpha One of the type I interferons produced by peripheral blood leukocytes or lymphoblastoid cells when exposed to live or inactivated virus, double-stranded RNA, or bacterial products. It is the major interferon produced by virus-induced leukocyte cultures and, in addition to its pronounced antiviral activity, it causes activation of NK cells. [NIH]... 

The sporadic failure of leukocyte cultures or occasional low mitotic rates in long-term stationary tissue cultures necessitates experimental replication, preferably on different days. Ideally, all concentrations and exposure times of the agent should be repeated in each experiment. In addition to replicate cultures for each experiment, replicate cytological observations are also important to eliminate possible sampling errors. To this end, multiple slides should be made from each culture and examined on different days. To overcome possible observer bias, the same cells should be evaluated by more than one investigator and the results compared. Such a system would allow comparisons of precision to be made by a single observer at different times as well as by two different observers. 

Lovett EJ, Lundy J (1977) The effect of thiabendazole in a mixed leukocyte culture. Transplantation 24 93-97... 

Because of the difficulty of treating a number of the sphingolipidoses, clinical biochemists have been very interested in the development of simple diagnostic tests which can also be used for heterozygous individuals. Fortunately, the enzyme deficiency is the same in all tissues (Table 12.6). Thus, easily available materials can be used for the assay of the enzymes concerned in each disease. Such material includes leukocytes, cultured skin fibroblasts, serum and hair follicles. Urine and tears have also been used. In most cases the enzymes are stable to freezing so that samples may be shipped to diagnostic centres where automated assays have been developed for many of the determinations (e.g. Peters et ai, 1975 cf. Brady, 1978). 

Propionic acidemia Leukocytes, cultured fibroblasts (for diagnosis), liver 232000... 

Proceedings of the Ninth Leukocyte Culture Conference, p. 727 (New York Academic... 

P. C. Nowell, Mitotic inhibition and chromosome damage by mitomycin in human leukocyte cultures, Exptl. Cell Res.33, 445-449 (1964). 

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