Inhibition lectin binding, effect

D. Page, D. Zanini, and R. Roy, Macromolecular recognition Effect of multivalency in the inhibition of binding of yeast mannan to concanavalin A and pea lectins by mannosylated dendrimers, Bioorg. Med. Chem., 4 (1996) 1949-1961. 

The deprotected lactosides were evaluated as inhibitors against lectin binding in a solid-phase inhibition assay with immobilized ASF on the surface of microtiter plate wells, mimicking cell-surface presentation, while mammalian galectins-1, -3, and -5 were in solution. Strong multivalency effects and selectivity were observed for the... 

In addition to the aforementioned effects on paracellular drug transport, Ca2+ also plays an important role in the transcytosis of macromolecules. The entry of the plant lectins abrin, modeccin, and viscumin into Vero cells was inhibited in a Ca2+-free medium a well as in a Ca2+-containing medium containing verapamil and Co2+, both inhibitors of Ca2+ [217], Ca2+ is therefore required at a stage after the binding of the above lectins, perhaps in the fusion and exocytosis of membrane vesicles. 

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