Kinases substrate specificity

MARCKS Myristolated, alanine-rich C kinase substrate specific protein kinase C substrate MBP Major basic protein MBSA Methylated bovine serum albumin... 

Proteins from cDNA libraries are used for measurement of kinase substrate specificity and identification of phospholipid-binding proteins. 

Palczewski, K. McDowell, J.H. Hargrave, P.A. Rhodopsin kinase substrate specificity and factors that influence activity. Biochemistry, 27, 2306-2313 (1988)... 

Tegge, W., Frank, R., Hofmann, F., and Dostmann, R.G. (1995) Determination of cyclic nucleotide-dependent protein kinase substrate specificity by the use of peptide libraries on cellulose paper. Biochemistry 34, 10569-10577. 

JH Till, RS Annan, SA Carr, WT Miller. Use of synthetic peptide libraries and phosphopeptide-selective mass spectrometry to probe protein kinase substrate specificity. J Biol Chem 269 7423-7428, 1994. 

Thymidine kinase Substrate specificity (gene therapy) 43-fold increase in sensitivity to gancydovir in hamster cells Cassette mutagenesis + selection + screening E. coli [85]... 

The a-interferon pathway is more complex and demonstrates how kinase substrate specificity can also play a role. 

The antiviral mechanism of action of triflnridine involves inhibition of viral DNA synthesis. Triflnridine monophosphate irreversibly inhibits thymidylate synthetase, and trifluridine triphosphate is a competitive inhibitor of thymidine triphosphate incorporation into DNA by DNA polymerases. Trifluridine is incorporated into viral and ceUnlar DNA. Trifluridine-resistant HSV with altered thymidine kinase substrate specificity can be selected in vitro, and resistance in clinical isolates has been described. 

INVESTIGATION OF THYLAKOID PROTEIN KINASE SUBSTRATE SPECIFICITY USING SYNTHETIC PEPTIDES. 

Investigation of Thylakoid Protein Kinase Substrate Specificity Using Synthetic Peptides 767... 

Crans DC, Whitesides GM. Glycerol kinase substrate specificity. J. Am. Chem. Soc. 1985 107 7008-7018. 

Lu R, Chan BS, Schuster VL. Cloning of the human kidney PAH transporter narrow substrate specificity and regulation by protein kinase C. Am J Physiol 1999 276(2 Pt 2) F295-F303. 

Szwergold, B.S., Howell, S., and Beisswenger, P.J. (2001) Human fructosamine-3-kinase purification, sequencing, substrate specificity, and evidence of activity in vivo. Diabetes 50, 2139-2147. 

Protein kinases differ in their cellular and subcellular distribution, substrate specificity and regulation 394... 

Protein kinases differ in their cellular and subcellular distribution, substrate specificity and regulation. These properties determine the functional roles played by the very large number of protein kinases that have been found in mammalian tissues, most of which are known to be expressed in neurons [3]. The major classes of protein serine-threonine kinase in the brain, listed in Table 23-1, are covered in this chapter. The major classes of protein tyrosine kinases in the brain are discussed in Chapter 24. 

Protein kinase C (PKC) comprises the other major class of Ca2+-dependent protein kinases and is activated by Ca2+ in conjunction with DAG and phosphatidylserine (discussed in Ch. 20). Multiple forms of PKC have been cloned, and the brain is known to contain at least seven species of the enzyme. The variant forms of PKC exhibit different cellular distributions in the brain and different regulatory properties. For example, they differ in the relative ability of Ca2+ and DAG to activate them some require both Ca2+ and DAG, whereas others can be activated by DAG alone, apparently without an increase in cellular Ca2+ concentrations. However, these enzymes show similar substrate specificities and, as a result, are often considered isoforms. 

Based on their substrate specificity, kinases have been divided into two broad classes serine/threonine- and tyrosine-specific kinases. Of the estimated 500 kinases, roughly one-fifth of these are tyrosine kinases. The two classes are further broken down and grouped by function, required cofactors, and substrates. ... 

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