Chromatography of amino acids ion exchange

Hamilton, P. B., Ion exchange chromatography of amino acids. A single column, high resolving, fully automatic procedure, Anal. Chem., 35, 2055, 1963. 

Murren, C., Stelling, D., and Felstead, G., An improved buffer system for use in single-column gradient-elution ion-exchange chromatography of amino acids, /. Chromatogr., 115, 236, 1975. 

PB Hamilton. Ion exchange chromatography of amino acids—microdetermination of free amino acids in serum. Ann NY Acad Sci 102 55 -75, 1962. 

El. Ertingshausen, G., and Adler, H. J., Fully-automated accelerated ion exchange chromatography of amino acids in physiologic fluids. Amer. J. Clin. Pathol. 53, 680-687 (1970). 

Hamilton P B. (1962) Ion exchange chromatography of amino acids Microdetermination of free ammo acids m serum Ann N Y Acad. Sa 102, 55- 75... 

Ion-exchange chromatography of amino acids on acid resins is a very efficient analytical procedure but is difficult to adapt to preparative separations. On the other hand, ion-exchange chromatography of acidic amino acids and peptides on basic resins is an efficient technique for preparative separations and is used mainly for that purpose. Thus, nearly all isolations of acid y-glutamyl derivatives from natural material have employed this technique. 

IsHii, S. Ion-exchange chromatography of amino acids and peptides. I. A new determination method of basic amino acids on a carboxylic acid resin. J. Biochem. (Tokyo) 43, 531—537 (1956). 

The number of each type of amino acid in a protein can be determined by acid hydrolysis and separation of the individual amino acids by ion exchange chromatography The amino acids are detected by colorimetric reaction with, for example, ninhydrin or fluorescamine. 

Chromatography, Ion Exchange, Determination of Amino Acids by Qacohs). 14 177... 

A crucial aspect of chromatography is the importance of minimizing the time needed for the solute to transit back and forth between the sorbent and liquid structures. This is necessary in order to obtain sharp peaks, as demonstrated by Martin and Synge in 19A1 (8). This experimental principle was adapted to the ion exchange separation of amino acids... 

The mixture of amino acids released by complete hydrolysis of lipopeptin A was fractionated by ion-exchange chromatography. Each amino acid was isolated and the optical rotation determined. Two moles of L-aspartic acid and 1 mole each of L-glutamic acid, L-serine, l-threonine, N-methyl-L-phenylalanine, L-threo-p-hydroxyglutamic acid and N-methyl-L-aspartic acid were obtained. 

In the ion-exchange technique, separated amino acids exiting (eluting) from the end of the chromatography column mix with a solution of ninhydrin and undergo a rapid reaction that produces an intense purple color. The color is detected by a spectrometer, and a plot of elution time versus spectrometer absorbance is obtained. 

Chin, C. C. Q., Ion-exchange chromatography of some amino acid derivatives found in proteins, Meth. Enzymol., 106, 17, 1984. 

Pfeifer RF and Hill DW (1983) High-performance liquid chromatography of amino acids ion-exchange and reversed-phase strategies. Adv Chromatogr 22,37-69. 

Dickenson JC, Rosenblum H, Hamilton PB (1965) Ion exchange chromatography of the free amino acids in the plasma of newborn infants. Pediatrics 36 2-13... 

K Stulik, V Pacakova, H Wang. High-performance liquid chromatography of amino acids and dipeptides on new ion exchangers of the HEMA series. J Chromatogr 552 439-448, 1991. 

Alkaline hydrolysis of cockroach residues subsequent to extraction under nitrogen with 80% ethanol yielded methionine and methionine sulfoxide in a ratio of 10 to 1. Additional evidence for the presence of combined methionine sulfoxide was obtained by measuring the amount of methionine sulfoxide-S35 in acid and enzymatic hydrolyzates after assimilation of Na2S3504. The data are believed to be indicative of naturally occurring peptide- or polysaccharide -bound methionine sulfoxide. Other combined amino acids were determined by ion exchange chromatography of the 5% trichloroacetic acid-insoluble cockroach residues after hydrolysis with acid or alkali. /3-Alanine, normally present only in the soluble fraction of an organism, was found in the insoluble, proteinaceous residue. 

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