Chromatography amino acid

Hei.ser, T., 1990. Amino acid chromatography The best technique for. student 2hs,. Journal of Chemical Education 67 964-966. 

The quantitative assay for PBG and ALA (Bio Rad, Hercules, CA, USA) that is based on the classical method by Mauzerall and Granick may be used for determination of the porphyrin precursors. PBG is absorbed by the anion-exchange column and ALA by the cation-exchange column interferences are washed out. After elution from the column, ALA is derivatized by acetyl acetone to form a pyrrole. Both ALA and PBG are determined colorimetrically with the modified Ehrlichs reagent. Instead of this broadly used standard method ALA, but not PBG may be detected and quantified using amino acid chromatography. However, our experience has shown that this method is only valid for detecting massively increased concentrations of ALA. 

SOLVENT SYSTEMS FOR DISYL-AMINO ACID CHROMATOGRAPHY ON SILICA GEL PLATES... 

D9. Dent, C. E., Clinical applications of amino acid chromatography. Scand. J. Clin, ir Lab. Invest. 10, Suppl. 31, 122-127 (1957). 

Interactions even weaker than ionic bonds can be used to separate enantiomers. Chromatographic separation relies on a difference in affinity between a stationary phase (often silica) and a mobile phase (the solvent travelling through the stationary phase, known as the eluent) mediated by, for example, hydrogen bonds or van der Waals interactions. If the stationary phase is made chiral by bonding it with an enantiomerically pure compound (often a derivative of an amino acid), chromatography can be used to separate enantiomers. 

Jl. Jackson, S. H., Sardharwalla, I. B., and Ebers, G. C., Two systems of amino acid chromatography suitable for mass screening. Clin. Biochem. 2, 163-167 (1968). 

P16. Perry, T. L., Stedman, D., and Hansen, S., A versatile lithium buffer elution system for single column automatic amino acid chromatography. J. Chromatogr. 38, 460-466 (1968). 

W3. Wadman, S. K., Fabery de Jonge, H., and de Bree, P. K., Rapid, high-resolution, two-dimensional amino acid chromatography on microscale chromatograms. Clin. Chim. Acta 26, 87-90 (1969). 

Originally devised to separate low-molecular-weight substances such as sugars and amino acids, chromatography has become an invaluable tool in protein purification. There are a wide variety of chromatographic techniques. They can be used to separate protein mixtures on the basis of molecular properties such as size, shape, and weight, or certain binding affinities. Often several techniques must be used sequentially to obtain a demonstrably pure protein. 

Lithium citrate buffers have been successfully used in automatic amino acid chromatography on resin columns. (See for example. Perry et al., 1968 Peters et al., 1968). 

Applegarth DA, Hardwick DF, Ross PM. Creatinine excretion in children and usefulness of creatinine equivalents in amino acid chromatography. Clin Chim Acta 1968 22 131-134... 

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