Eukaryotes intracellular contents

Eukaryotes in early evolution must have been subjected to wide variations in environmental temperature and water activity. Probably intracellular water was also involved. For example, fluxes in water across the plasmalemma would force the cell to cope with changes in water content in order to ensure that metabolism would not shut down. Metabolic stability would certainly be the selective factor. 

Sohn and co-workers reported a novel device which can quantify the DNA content within the nucleus of single eukaryotic cells [3]. Since DNA molecules are highly charged in intracellular environment, they will be polarized in an applied low-frequency AC electric field. 

Sohn and co-workers reported a novel device which can quantify the DNA content within the nucleus of single eukaryotic cells [3]. Since DNA molecules are highly charged in intracellular environment, they will he polarized in an applied low-frequency AC electric field. This polarization response can he measured as a change in total capacitance ACj, across a pair of microelectrodes as individual cells suspended in buffer solution flow one by one through a microchannel (as shown in Fig. 1 and Fig. 2). They found that there is a linear relationship between the capacitance and the DNA content of a cell. And they further showed that this relationship is not species-dependent. This innovative technique is termed as capacitance cytometry, which helps to reveal changes in cellular internal properties and determine the phase of individual cell in cell-cycle. 

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