Trenbolone acetate

Therapeutic Function Anabolic steroid Chemical Name 17j3-Aceto-3oxoestra-4,9,11-triene-3one Common Name Trienolone acetate Structural Formula  

Trade Name Manufacturer Country Year Introduced 

17 S-Benzyloxy-4,5-seco-estra-9,11-diene-3,5-dione Sodium-t-amylate Acetic acid i Methanol 

Stage A Preparation of 17 -Bemoyloxy-Estra-4, 11-Trien-3-one — 0.400 g of 17j3-benzoyl-oxy-4,5-seco-estra-9,11 -diene-3,5-dione is dissolved in 4 cc of toluene under an inert atmosphere. The solution is cooled to 3°C, then 0.48 cc is added of the solution of sodium tert-amyl-ate in anhydrous toluene, diluted by the addition of a further 4.8 cc of anhydrous toluene. 

This reaction mixture is kept between 0°C and +5°C tor six hours, with agitation and under an inert atmosphere, then 5 cc of a 0.2 N solution of acetic acid in toluene are added. The mixture is extracted with toluene, and the extracts are washed with water and evaporated to dryness. The residue is taken up in ethyl acetate, and then the solution is evaporated to dryness in vacuo, yielding a resin which is dissolved in methylene chloride, and the solution passed through a column of 40 g of magnesium silicate. Elution Is carried out first with methylene chloride, then with methylene chloride containing 0.5% of acetone, and 0.361 g is thus recovered of a crude product, which is dissolved in 1.5 ccof Isopropyl ether then hot methanol is added and the mixture left at 0°C for one night. 

324 g of the desired 1 7/3-benzoYloxy-estra-4,9,11 -trien-3-one are thus finally obtained, being a yield of B5%, melting point is 1 54°C. 

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One possible mechanism responsible for the abiHty of trenbolone acetate to stimulate skeletal muscle hypertrophy may be through enhanced proliferation and differentiation of satelHte ceUs as the result of increased sensitivity to insuHn-Hke growth factor-I (IGE-1) and fibroblast growth factor (43). 

C25H27CIO3 /O/Oy-i -J) see Trenbolone acetate ( )-2-chioro-3-[4-[(6-hydroxy-2,5,7,8-tetramethyl-chroman-2-yl)incthoxy]phenyl]propionic acid (C23H27C10 97322-69-5) sec Troglitazone 4 -chloro-2 (24midazoUn-2-yl)benzophenone (Ci HjiClNjO 22590-J7-6) see Mazindo ... 

Estradiol-17 is given to animals in form of a subcutaneous implant in the ear, alone or in combination with other hormonally active compounds such as progesterone or trenbolone acetate. Estradiol-17 is used in steers, to best advantage, but also exhibits some anabolic effects in heifers and veal calves. It works best in lambs in conjunction with androgens, but is not effective as an anabolic agent in pigs. It has been used in many forms in the past including the benzoate, dipropionate, hemisuccinate, heptanoate, propionate, undecanoate, and valerate esters. 

Trenbolone acetate is a synthetic steroid with hormonal activity similar to testosterone but with greater anabolic activity. After administration to cattle, trenbolone acetate is rapidly hydrolyzed to its free hydroxylated form (28). The 17 -OH epimer is the major metabolite occurring in the excreta, bile, and liver. 

Following implantation of 200 mg of radiolabeled trenbolone acetate in calves and heifers, maximum levels of residues in tissues occurred at about 30 days postimplantation (31). The highest total drug-related residues expressed as trenbolone equivalents were approximately 50 and 3 ppb in liver and muscle, respectively. Only 25% and 10% of those residues could be extracted by ether or ethyl acetate from glucuronidase-treated liver and muscle samples, respectively. Tire majority of trenbolone residues were not extractable by organic solvents, a finding suggesting that they were covalently bound to tissues (32). 

Unlike the naturally occurring hormones for which human food safety assessment is based on a ratio of their amount consumed in food compared to their amount produced endogenously by the consumer, safety assessment for the synthetic trenbolone acetate and zeranol hormones is based on alternative... 

Trenbolone acetate -Trenbolone (muscle) Cattle Muscle 2 0-0.02 (0 0... 

Trenbolone acetate Sum of trenbolone acetate and 17 alpha-, and 17 betatron bolone, both free and conjugated, expressed as trenbolone Cattle, pig... 

In June 1987, the JECFA established acceptable daily intake levels and acceptable residue limits for zeranol and trenbolone acetate and decided that these levels were not needed for the naturally occurring hormones. With respect to the natural hormones, the JECFA explained that residues resulting from use of these... 

The same situation applies to testosterone and progesterone. Unlike the naturally occurring hormones, there is no daily production rate for the synthetic compounds trenbolone acetate and zeranol. Therefore, the FDA requires toxicological testing in animals to determine a safe level in meat for these compounds. When FDA approved zeranol and trenbolone, it determined that residues were well below a safe concentration and therefore no residue tolerance was required (25). Due to consumer concerns, testing procedures for zeranol were put into place in the early 1980s but were discontinued in the late 1980s because no residue violations were found. 

In Kuwait, a total of 350 samples including 230 sheep urine, 30 beef meat, and 90 chickens were screened for the presence of residues of trenbolone acetate (40). The results obtained showed that the trenbolone acetate levels in the urine ranged from 0.1 to 0.9 ppb and in the muscle tissue from 0.02 to 0.05 ppb, none of the figures exceeding the MRL of 2 ppb set by the Food and Agriculture Organization/ World Health Organization (FAO/WHO). 

MGA) (4) testosterone (5) propionate [57-85-2] in combination with estradiol benzoate, ie, Synovex-H or Heifer-oid and a synthetic testosterone analogue, trenbolone acetate [10161 -34-9] (TBA) (6) which is used alone, ie, Finaplix, or in combination with estradiol, ie, Revalor. Structures of these anabolic steroids are shown in Figure 1. 

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