Automated imaging

The specific advantage of this technique is its higher sensitivity than classical film radiography and the potential for automated image processing due to the digital read-out of the IP s. Former investigations have shown / / that the available IP s achieve the same contrast like NDT films with an IP type and scanner dependent unsharpness. 

Computer-assisted molecular modeling package, 10 331-333 Computer-assisted tomography (CAT), sodium iodide in, 22 827 Computer-automated image analysis, as a particle counting method, 18 146-149 Computer-controlled viscometers, 21 732 Computer controller, closed loop fuel metering system, 10 57 Computer control loop, components of, 20 677-678... 

A new suspension array concept based on sedimentation and microscopic imaging was introduced by Moser et al. [98], Magnetic microbeads settle to the bottom of a microplate well by magnetic forces and form randomly ordered arrays, which are examined by fluorescence microscopy and automated imaging analysis. Each bead carries specific capture molecules and can be identified by a defined luminescent code. 

Ribeiro E, Stafslien SJ, Casse F, Callow JA, Callow ME, Pieper RJ, Daniels JW, Bahr JA, Webster DC (2008) Automated image-based method for laboratory screening of coating libraries for adhesion of algae and bacterial biofilms. J Comb Chem 10 586-594... 

Some of the newly created screening centers were equipped with automated imagers and HCS entered the academic field. In general, these academic screening centers were well funded and could afford the prices demanded by vendors, perpetuating the tradition of cost-intensive screening. 

CellProfiler and CellProfiler Analyst are free Open Source software for automated image analysis, data visualization, and machine learning. Versions for Mac, Windows, and Linux are available and both software can be downloaded at http //www.cellprofiler. org. CellProfiler was developed by Anne Carpenter and Thouis Jones in the laboratory of David Sabatini at the Whitehead Institute for Biomedical Research and by Polina Golland at the CSAIL of the MIT. 

Santos AF, Zaltsman AB, Martin RC et al (2008) Angiogenesis an improved in vitro biological system and automated image-based workflow to aid identification and characterization of angiogenesis and angiogenic modulators. ASSAY Drug Devel Technol 6 693-710... 

Variable migration across the invasion barrier if not carefiiUy controUed for thickness and reproducibility of protein batches laborious unless using fluorescent cells and automated imaging... 

Although all the methods mentioned above can generate reproducibly sized tumor spheroids, the one that we recommend is the method that uses round-bottom ultralow attachment 96-well plates (11). This method is the least time consuming (e.g., plates do not require any coating), reproducible (one centrally located spheroid/well is formed), and suitable for automated imaging. Thus it satisfies important requirements of cell-based HT assays. 

Quantitative analysis of these images is accomplished in the same manner as for any microscope image, depending on the detail of analysis desired. Automated image analysis software can be used to provide a variety of information. Crystal phase volume (solid fat content UNIT D3.i) can be obtained by counting dark pixels (according to some empirical threshold factor). 

The first step of automated image analysis of microorganisms is the visualization. Considering their size range, microscopes (or for the largest objects, microviewers) have to be used. Two aspects should be discussed ... 

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