Immunological analysis

Wang D Jiang X., Chen P., Inouchi J et al. (1993). Chemical and immunological analysis of prey-derived vomeronasal stimulants. Brain Behav Evol 41, 246-254. 

J Choi, C Kim, MJ Choi. Immunological analysis of metamphetamine antibody and its use for the detection of metamphetamine by capillary electrophoresis with laser-induced fluorescence. J Chromatogr 705 277-282, 1998. 

Lefranc, M. R, Hammarstrom, L., Smith, C. I. E., and Lefranc, G., Gene deletions in the human immunoglobulin heavy chain constant region locus Molecular and immunological analysis. Immunol. Rev. 2, 265-281 (1991). 

Hosoda H, Ishikawa E (1993) Coupling of haptens to carriers and to labels. In Masseyeff RF, Albert WH, Staine NA (eds.) Methods of immunological analysis. Samples and reagents. VCH, Weinheim, p... 

As with other kinds of glycoproteins, physical adsorption is an easy way to attach the antibodies to solid surfaces, since no reagents or modification are required. These features have promoted extensive use of adsorption as immobilization methodology in immunological analysis. The adsorption has been performed on different surface, mainly... 

For all the aforementioned reasons, it is possible to conclude that GEC-based platforms are very suitable for the immunological analysis of food residues according to the food management programs on Hazard Analysis Critical Control Point (HACCP). Procedures 33 and 34 (see in CD accompanying this book) give experimental details on the detection of pesticide atrazine and sulfonamide residues in food samples. 

Microfluidic-based electrochemical platform for rapid immunological analysis in small volumes... 

Neumann, H. and Mullner, S. (1998) Two replica blotting methods for fast immunological analysis of common proteins in two-dimensional electrophoresis. Electrophoresis 19, 752-757. 

Depending on the research question, other organs such as spleen and lymph nodes can be removed from the mice for (immunological) analysis, fixed in 4% formalin, and subsequently in 70% ethanol before paraffin embedding. Alternatively, the tissue, placed in aluminum cryotubes, can be snap frozen immediately in liquid nitrogen after dissection. This procedure should be performed if RNA, DNA, or protein needs to be isolated from the tissue. 

M24. Mirro, J., Jr., Kitchingman, G., Behm, F. G., Murphy, S. B., and Goorha, R. M., T cell differentiation stages identified by molecular and immunologic analysis of the T cell receptor complex in childhood lymphoblastic leukemia. Blood 69,908-912 (1987). 

Immunologic analysis Determine the peripheral blood cell counts and differentials. Additionally, lymphoid cells are analyzed for weight and cell number in the... 

Centrifugal Fast Analyzer for Biochemical and Immunological Analysis, The Use of... 

Majumder, A., Duttagupta, S., Goldwasser, P., Donahue, T., and Henry, S., 1981, The mechanism of interallelic complementation at the INOl locus in yeast Immunological analysis of mutants. Mol. Gen. Genet. 184 347-354. 

Petteway, Jr., S.R., Reed, D.L., Tribe, D.E., and Kennedy, W.R. (1989). HIV recombinant proteins immunological analysis and applications as reagents for serological tests. In HIV Detection by Genetic Engineering Methods. P.A.Luciw and K.S.Steimer, eds. (New York Marcel Dekker), pp. 59 76. 

The test strips or slides cannot be calibrated with aqueous solutions (primary calibrator). This is because the matrix of the sample and of the primary calibrator is not the same. Calibration with so-called secondary calibrators is an alternative. The concentration or activity of the intended analyte is determined by chemical, enzymatic, physical or immunological analysis in any case it should be obtained by the best available method. 

Quantitative immunological analysis indicated that the insecticidal protein toxin constituted approximately 1% of the total protein in 112-12RN and Ps3732RN when the B.t. gene was in the same orientation as the Km gene. Placement of the B.t. gene in the opposite orientation to the Km gene resulted in lower levels of protein production, estimated to constitute 0.1% of the total protein of the cell. 

Suzuki, T., Coggan, M., Shaw, D. C., and Board, P. G., Electrophoretic and immunological analysis of human glutathione S-transferase isoenzymes. A/m. Hum. Genet 51,95-106(1987). Suzuki, T., Shaw, D. C., and Board, P. G., Purification and characterization of acidic glutathione S-transferase 6 from human brain. Biochem. J. 274, 405-408 (1991). 

Cl. Cell, P. G. H., Immunological analysis of abnormalities in the human serum protein by a gel-diffusion method. /. Clin. Pathol. 8, 269 (1955). 

Slater, R. J., The serum globulins defined by electrophoretic and immunologic analysis. Arch. Biochem. Biophys. 69, 33-44 (1955). 

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