Serum proteins, human

Figure 3. Fractionation of human serum proteins on Spherogel TSKSW 3000, The conditions were as in Figure 1. The analyses were made using (A) a 50-fiL injection loop with an analytical flow cell (B) a 100-L loop with a semipreparative flow cell or (C,D) a 500-L loop with a preparative flow cell.

Hall, L. M Hall, L H Kier, L B. QSAR modeling of 3-lactam binding to human serum proteins. /. Comput.-Aided Mol. Des. 2003, 17,103-118. 

Cooper JR, Gowing HS. 1981. The binding of americium and curium to human serum proteins. IntJ Radiat Biol 40(5) 569-572. 

L5. Laurell, A.-B., Inhibitor capacity of some purified human serum proteins on hemagglutination by influenza virus. Acta Pathol, et Microbiol. Scand. 49, 213 (1960). 

The pharmacokinetics of minaprine (orally administered) in humans have been studied [ 146,147]. The bioavailability has been found to be excellent, and the pharmacokinetic parameters did not show a dose-dependency [147]. The binding of (41) to human serum proteins and erythrocytes has been investigated [148]. Minaprine has been found to undergo extensive biotransformation in different species, with major groups of metabolites derived from degradation of the morpholine subunit and from hydroxylation of the phenyl substituent [149-152],... 

Figure 3.25 Starch gel electrophoresis of human serum proteins. Samples 1 and 2 are normal while samples 3, 4 and 5 show an extra band adjacent to the normal albumin which is due to the presence of bis-albumin. Sample 7 contains a myeloma protein that has remained near the origin. (Photograph by permission of Dr D. Brocklehurst, Department of Clinical Chemistry, Doncaster Royal Infirmary, UK.)...

Figure 11.13 Electrophoresis of human serum proteins. The electrophoretogram, after staining with a suitable dye, can be scanned using a densitometer, which gives a trace of the absorbance pattern of the strip.

Figure 11.15 Immunoelectrophoresis of human serum proteins. The proteins are separated electrophoretically from wells cut in a suitable gel. After electrophoresis, a trough is cut in the gel parallel to the direction of migration and filled with an antiserum. The components are allowed to diffuse for 24-48 hours for precipitation lines to develop. Human serum contains many proteins, among which the immunoglobulins can be identified.

James, K. and Stanworth, D. R. (1964) Studies on the chromatography of human serum proteins on diethylaminoethyl(DEAE)-cellulose. (1) The effect of the chemical and physical nature of the exchanger. J. Chromatog. 15, 324-335. 

Distribution - Argatroban has an apparent steady-state volume of distribution is 174 mL/kg. It is 54% bound to human serum proteins. 

Binding of entecavir to human serum proteins in vitro was approximately 13%. 

Absorption/Distribution - Atazanavir is rapidly absorbed, with a T ax approximately 2.5 hours in healthy subjects (2 hours in HIV-infected patients). Steady state is achieved between days 4 and 8. Atazanavir is 86% bound to human serum proteins. 

PA McDonnell, GW Caldwell, JA Masucci. Using capillary electrophoresis/ frontal analysis to screen drugs interacting with human serum proteins. Electrophoresis 19 448-454 (1998). 

Ldnnerdal, B., Stanislowski, A. G., Hurley, L. S. J. Inorg. Biochem. 12, 71 (1980) Woittierz, J. R. W. Elemental Analysis of Human Serum and Human Serum Protein Fractions by Thermal Neutron Activation, Netherlands Energy Research Foundation Report, ECN 147, January 1984... 

Davis, B.J. 1964. Disc electrophoresis II Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121 404-427. 

Figure 6.10 Binding of toxicants to blood proteins (a) Double-reciprocal plot of binding of rat serum lipoprotein fraction with four insecticides. Insert illustrates magnitude of differences in slope with Scatchard plot, (b) Scatchard plot of binding of salicylate to human serum proteins. (Sources (a) Skalsky and Guthrie, Pest. Biochem. Physiol. 7 289, 1977 (b) Moran and Walker, Biochem. Pharmacol. 17 153, 1968.)...

Magi, B., Marzocchi, B., Bini, L., Cellesi, C., Rossolini, A., and Pallini, V. (1995) Two-dimensional electrophoresis of human serum proteins modified by ampicillin during therapeutic treatment. Electrophoresis 16, 1190-1192. 

S. Glasson, R. Zini, P. D Athis, J. P. Tillement, J. R. Boissier, The distribution of bound propranolol between the different human serum proteins, Mol. Pharmacol. 77 187-191 (1980). 

Wl. Wahab, E., Adjutantis, G., and Laurence, D. J. R., Borate buffer as a medium for the ionophoresis of human serum proteins. Biochem. J. 60, Proc. xiii (1955). 

A rapid two-dimensional separation of a microspot of protein solution under high field intensity is a new application of the two-dimensional principle. The various fractions emerge as a series of rays out of the application zone and hence the designation star electrophoresis which was introduced by us in connection with work on human serum proteins (P4). A similar discontinuous procedure was already mentioned by Strain in 1951 (S4, S5) for the separation of metal ions and recently used by Pucar (P8) and applied by Keler et al. (K1) to human serum. 

L Bini, B Magi, B Marzocchi, C Cellesi, B Berti, R Raggiaschi, A Rossolini, V Pallini. Two-dimensional electrophoretic patterns of acute-phase human serum proteins in the course of bacterial and viral diseases. Electrophoresis 17 612-616,1996. 

Post-separation labeling was also achieved for separation of four human serum proteins (IgG, transferrin, al-antitrypsin, and albumin) using 0.2 mM of TNS. This is a virtually non-fluorescent reagent which, upon non-covalent association with proteins, produces a fluorescent complex (A= 325 nm, A = 450... 

Heimburger, K., K. Heide, H. Haupt, and H. E. Schultze Building stones of human serum protein. Clin. Chem. Acta 10, 293 (1964). 

Basic amino acid content of human serum proteins. Influence of the ingestion of arginine on the composition of the serum proteins. Ibid., 133, 71 (1940). 

Amino acid composition of the serum proteins. II. Fractionation of human serum proteins by cellulose ion-exchange chromatography and comparative amino acid composition of the fractions. Ibid., 19, 451 (1958). With S. Keller. 

FPLC-ICP-MS Serum Al bound to proteins Separation of human serum proteins was achieved on an anion-exchange column coupled with a spectrophotometric detector for proteins and a ICP-MS for Al [84]... 

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