Centrifugal fast analyzer

Burtis, Carl A, Tiffany, Thomas O., and Scott, Charles D., The Use of a Centrifugal Fast Analyzer for Biochemical and Immunological... 

Centrifugal Fast Analyzer for Biochemical and Immunological Analysis, The Use of... 

Scott CD, Burtis CA, Johnson WF, Thacker LH, Tiffany TO. A small portable centrifugal fast analyzer system. Clin Chem 1974 20 1003-8. 

Parallel Fast. Parallel-fast analyzers are a special variation of batch analyzers, based on the use of a centrifuge. They are sometimes called centrifugal fast analyzers. The principle is illustrated in Figure 24.13. A central, removable... 

Figure 24.13. Cross-section of sample disc and centrifugal-analyzer section of parallel-fast analyzer. From R. C. Coleman, W. D. Schultz,...

Protein precipitation is used routinely in bioanalytical laboratories in the pharmaceutical industry. Plasma is mixed with an excess (3 to 5 times) of organic solvent (typically acetonitrile or methanol) or an acid such as formic acid. The proteins precipitate out of solution, the sample is centrifuged, and the supernatant is analyzed. While this method is relatively fast and simple, the extract contains salts and lipids that can interfere with subsequent analyses. Often, a second technique such as SPE is used for further cleanup. Table 2.4 exhibits various samples that... 

Blood samples are drawn after an overnight fast of at least 12 h. Preheparin and postheparin plasma samples are collected to analyze the LPL activity. In general 50-100 IU/kg heparin is injected intravenously and the postheparin plasma sample is collected 15 min later [41]. Samples should be immediately centrifuged and the plasma should be directly analyzed or frozen at - 70°C. 

By comparison, the HOST centrifugation technique (Section 8.4.2.1) is much more operator friendly and therefore is attractive for both R D and production applications. It can measure relatively concentrated emulsions, which may be an important advantage in cases where an emulsion becomes coUoidaUy imstable upon dilution. A potentially significant disadvantage of this technique is that one measurement may require several hours of centrifugation, making it less useful in production situations where fast decisions are required. On the other hand, in R D environments it can prove to be very attractive, since multiple samples (e.g. 12) can be analyzed at the same time. 

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