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Immuno-PCR

Carcinoembryonic antigen (CEA) 2001 A Circulating CEA in human sera 92.3% of patients with high CEA levels and 50% of patients with normal CEA levels were found positive Ren et al. [18], use of a bispecific CEAScFv-streptavidin fusion protein-based immuno-PCR technique (see Section 2.1.1)... [Pg.242]

For any standard ELISA application requiring enhanced sensitivity in combination with almost complete retaining of all ELISA advantages, such as microplate format, common laboratory equipment and the immunological specificity for target antigens, however, Immuno-PCR is worth consideration. [Pg.285]

Sano T, Smith CL, Cantor CR. Immuno-PCR Very sensitive antigen detection by means of specific antibody-DNA conjugates. Science 1992 258(5079) 120-122. [Pg.286]

Schiavo S, El-Shafey A, Jordan N, Orazine C, Wang Y, Zhou X, Chiu NH, Krull IS. Pushing the limits of detection with immuno-PCR. PharmaGenomics 2004 36-44. [Pg.286]

Quijada L, Moreira D, Soto M, Alonso C, Requena JM. Detection of major histocompatibility complex class I antigens on the surface of a single murine blastocyst by immuno-PCR. BioTechniques 1997 24(4) 660-662. [Pg.286]

Ren J, Ge L, Li Y, Bai J, Liu WC, Si XM. Detection of circulating CEA molecules in human sera and leukopheresis of peripheral blood stem cells with E. coli expressed bispecific CEAScFv-streptavidin fusion protein-based immuno-PCR technique. Ann NY Acad Sci 2001 945 116-118. [Pg.286]

Zhou H, Fisher RJ, Papas TS. Universal immuno-PCR for ultra-sensitive target protein detection. Nucleic Acids Res 1993 21(25) 6038-6039. [Pg.287]

Kakizaki E, Yoshida T, Kawakami H, Oseto M, Sakai T, Sakai M. Detection of bacterial antigens using immuno-PCR. Lett Appl Microbiol 1996 23(2) 101-103. [Pg.287]

Niemeyer CM, Adler M, Pignataro B, Lenhert S, Gao S, Chi L, Fuchs H, Blohm D. Self-assembly of DNA-streptavidin nanostructures and their use as reagents in immuno-PCR. Nucleic Acids Res 1999 27(23) 4553-4561. [Pg.287]

Mweene AS, Ito T, Okazaki K, Ono E, Shimizu Y, Kida H. Development of immuno-PCR for diagnosis of bovine herpesvirus 1 infection. J Clin Microbiol 1996 ... [Pg.287]

Case MC, Burt AD, Hughes J, Palmer JM, Collier JD, Bassendine MF, Yeaman SJ, Hughes MA, Major GN. Enhanced ultrasensitive detection of structurally diverse antigens using a single immuno-PCR assay protocol. J Immunol Methods 1999 223(1) 93—106. [Pg.287]

Maia M, Takahashi H, Adler K, Garlick RK, Wands JR. Development of a two-site immuno-PCR assay for hepatitis B surface antigen. J Virol Methods 1995 ... [Pg.287]

Sanna PP, Weiss F, Samson ME, Bloom FE, Pich EM. Rapid induction of tumor necrosis factor alpha in the cerebrospinal fluid after intracerebroventricular injection of lipopolysaccharide revealed by a sensitive capture immuno-PCR assay. Proc Natl Acad Sci USA 1995 92(l) 272-275. [Pg.287]

Saito K, Kobayashi D, Sasaki M, Araake H, Kida T, Yagihashi A, Yajima T, Kameshima H, Watanabe N. Detection of human serum tumor necrosis factor-alpha in healthy donors, using a highly sensitive immuno-PCR assay. Clin Chem 1999 45(5) 665-669. [Pg.287]

Furuya D, Kaneko R, Yagihashi A, Endoh T, Yajima T, Kobayashi D, Yano K, Tsuda E, Watanabe N. Immuno-PCR assay for homodimeric osteoprotegerin. Clin Chem 2001 47(8) 1475-1477. [Pg.287]

Niemeyer CM, Adler M, Blohm D. Fluorometric polymerase chain reaction (PCR) enzyme-linked immunosorbent assay for quantification of immuno-PCR products in microplates. Anal Biochem 1997 246(1) 140-145. [Pg.288]

Komatsu M, Kobayashi D, Saito K, Furuya D, Yagihashi A, Araake H, Tsuji N, Sakamaki S, Niitsu Y, Watanabe N. Tumor necrosis factor-alpha in serum of patients with inflammatory bowel disease as measured by a highly sensitive immuno-PCR. Clin Chem 2001 47(7) 1297-1301. [Pg.288]

Case M, Major GN, Bassendine MF, Burt AD. The universality of immuno-PCR for ultrasensitive antigen detection. Biochem Soc Trans 1997 25(2) 374S. [Pg.288]

Wu HC, Huang YL, Lai SC, Huang YY, Shaio MF. Detection of Clostridium botulinum neurotoxin type A using immuno-PCR. Lett Appl Microbiol 2001 32(5) 321—325. [Pg.288]

McKie A, Samuel D, Cohen B, Saunders NA. Development of a quantitative immuno-PCR assay and its use to detect mumps-specific IgG in serum. J Immunol Methods 2002 261(1-2) 167-175. [Pg.288]

Ruzicka V, Marz W, Russ A, Gross W. Immuno-PCR with a commercially available avidin system [letter]. Science 1993 260(5108) 698-699. [Pg.289]

Niemeyer CM, Wacker R, Adler M. Combination of DNA-directed immobilization and immuno-PCR Very sensitive antigen detection by means of self-assembled DNA-protein conjugates. Nucleic Acids Res 2003 31(16) e90. [Pg.289]

Adler M, Wacker R, Niemeyer CM. A real-time immuno-PCR assay for routine ultrasensitive quantification of proteins. Biochem Biophys Res Commun 2003 308(2) 240-250. [Pg.289]

Chye SM, Lin SR, Chen YL, Chung LY, Yen CM. Immuno-PCR for detection of antigen to Angiostrongylus cantonensis circulating fifth-stage worms. Clin Chem 2004 50 (1) 51—57. [Pg.289]


See other pages where Immuno-PCR is mentioned: [Pg.402]    [Pg.296]    [Pg.239]    [Pg.240]    [Pg.241]    [Pg.248]    [Pg.249]    [Pg.251]    [Pg.253]    [Pg.255]    [Pg.257]    [Pg.259]    [Pg.261]    [Pg.265]    [Pg.267]    [Pg.271]    [Pg.273]    [Pg.275]    [Pg.277]    [Pg.279]    [Pg.281]    [Pg.281]    [Pg.283]    [Pg.285]    [Pg.287]    [Pg.289]   


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