Imaging digital microscopy

Digital Imaging Flnorescence Microscopy Spatial Heterogeneity of Photobleaching Rate Constants in Individual Cells, T. Cell. Biol.. 1985, 100, 1309-1322,... 

Benson, D., Bryan, J., Plant, A., Gotta, A. J. and Smith, L. (1985). Digital imaging fluorescence microscopy Spatial heterogeneity of photobleaching rate constants in individual cells. J. Cell Biol. 100, 1309-23. 

Digital microscopy is used to extract quantitative information from microscopic slides. A digital microscopic imaging system consists of the following six components (Huang, 1981b) ... 

Wilkinson MHF (1998) Optical systems for image analysed microscopy In Wilkinson MHF, Schut F (eds) Digital Image Analysis of Microbes. Wiley, New York, pp 431... 

Smith MW, Miyashita M, Phelps PC, et al. 1989. The effect of formaldehyde on cytosolic Ca of transformed human bronchial epithelial cells as measured by digital imaging fluorescence microscopy. Proc Am Assoc Cancer Res 30 107. 

Sluder, Greenfield, and D. E. Wolf, eds. Digital Microscopy. 3d ed. Boston Elsevier Academic Press, 2007. A guide to coordinating microscopes with digital cameras to capture and analyze microscopic images. Includes detailed laboratory exercises to demonstrate principles in action. 

Szucs, S. Vamosi, G. Poka, R. Sarvary, A. Bardos, H. Balazs, M. Kappelmayer, J. Toth, L. Szollosi, J. Adany, R. Single-cell measurement of superoxide anion and hydrogen peroxide production by human neutrophils with digital imaging fluorescence microscopy. Cytometry 1998, 33, 19-31. 

Analytical electron microscopy permits structural and chemical analyses of catalyst areas nearly 1000 times smaller than those studied by conventional bulk analysis techniques. Quantitative x-ray analyses of bismuth molybdates are shown from lOnm diameter regions to better than 5% relative accuracy for the elements 61 and Mo. Digital x-ray images show qualitative 2-dimensional distributions of elements with a lateral spatial resolution of lOnm in supported Pd catalysts and ZSM-5 zeolites. Fine structure in CuLj 2 edges from electron energy loss spectroscopy indicate d>ether the copper is in the form of Cu metal or Cu oxide. These techniques should prove to be of great utility for the analysis of active phases, promoters, and poisons. 

Jovin, T. and Arndt-Jovin, D. (1989). FRET microscopy Digital imaging of fluorescence resonance energy transfer. Application in cell biology. In Cell Structure and Function by Microspectrofluorometry (Kohen, E., JG, H. and Ploem, J., eds.). Academic Press, London, pp. 99-117. 

Jovin, T. and Arndt-Jovin, D. (1989). Luminescence digital imaging microscopy. Ann. Rev. Biophys. Chem. 18, 271-308. 

Nagy, P., Vamosi, G., Bodnar, A., Lockett, S. J. and Szollosi, J. (1998). Intensity-based energy transfer measurements in digital imaging microscopy. Eur. Biophys. J. 27, 377-89. 

There has been a continued interest in examining the properties of intact living cells using fluorescence microscopy. This field has seen considerable advances since the application of digital imaging techniques. In examining whole cells, one has to be especially aware of the location(s) of the probe. This is particularly important when bulk measurements are to be made on intact cells. 

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