Fluorescence digital imaging microscopy

D. J. Amdt-Jovin et al. Fluorescence Digital Imaging Microscopy in Cell Biology, Science (Washington D.C.) 230 (1985) 247 - 256. 

The application of in situ hybridization (ISH) has advanced from short lived, non-specific isotopic methods, to very specific, long lived, multiple color fluorescent-ISH probe assays (FISH). Improvements in the optics, filter technology, microscopes, cameras, and data handling by software, have allowed for a cost effective FISH setup to be within reach of most researchers. The application of mFISH (multiplex-FISH), coupled to the advances in digital imaging microscopy, have vastly improved the capabilities for non-isotopic detection and analysis of multiple nucleic acid sequences in chromosomes and genes (1). 

Ried, T., Baldini, A., Rand, T. C., and Ward, D. C, (1992) Simultaneous visualization of seven different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy. Proc. Natl. Acad. Sci. USA 89, 1388-1392. 

Jovin, T. and Arndt-Jovin, D. (1989). FRET microscopy Digital imaging of fluorescence resonance energy transfer. Application in cell biology. In Cell Structure and Function by Microspectrofluorometry (Kohen, E., JG, H. and Ploem, J., eds.). Academic Press, London, pp. 99-117. 

Benson, D., Bryan, J., Plant, A., Gotta, A. J. and Smith, L. (1985). Digital imaging fluorescence microscopy Spatial heterogeneity of photobleaching rate constants in individual cells. J. Cell Biol. 100, 1309-23. 

There has been a continued interest in examining the properties of intact living cells using fluorescence microscopy. This field has seen considerable advances since the application of digital imaging techniques. In examining whole cells, one has to be especially aware of the location(s) of the probe. This is particularly important when bulk measurements are to be made on intact cells. 

Slavik J (1998) Single and multispectral parameter fluorescence microscopy. In Wilkinson MHF, Schut F (eds) Digital Image Analysis of Microbes. Wiley, New York,... 

Smith MW, Miyashita M, Phelps PC, et al. 1989. The effect of formaldehyde on cytosolic Ca of transformed human bronchial epithelial cells as measured by digital imaging fluorescence microscopy. Proc Am Assoc Cancer Res 30 107. 

The phase behaviour of phospholipid monolayers at electrolyte/gas interfaces is studied by fluorescence microscopy. At the LE/LC phase transition, phase separation leads to a WignerH ype lattice structure. The observations are quantified using digital image processing. The results show that the phase transition comprises three different regimes. 

Figure 10, A) Micrograph by iriple exposure of color slide film using conventional fluorescence microscopy B) Pseudocolored digital image recorded with the CCD camera for chromosome 1.2,7, 8, 9. 12 and the human X chromosome Green = F1TC Red = TRlTC Blue = DAPI (Courtesy of T. Cremer. Heidelberg, Germany and Oxford University Press)...

Szucs, S. Vamosi, G. Poka, R. Sarvary, A. Bardos, H. Balazs, M. Kappelmayer, J. Toth, L. Szollosi, J. Adany, R. Single-cell measurement of superoxide anion and hydrogen peroxide production by human neutrophils with digital imaging fluorescence microscopy. Cytometry 1998, 33, 19-31. 

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