Glycosphingolipids profiling

Glycosphingolipid profiling deals with the structural issues of determining both the carbohydrate and Upid portions. Carbohydrate chains can be either hnear or branched, and can reach up to 50 monosaccharide units in length. Moreover, they can be substituted by rather labile siaHc acids or fucose at different locations. The lipid portion is heterogeneous in the sphingosme base moiety, and can contain different lengths of fatty acids hnked by an amide bond. 

Nojiri, H., Takaku, H., Tetsuka, T, Motoyoshi, K., Miura, Y, and Saito, M., 1984, Characteristic expression of glycosphingolipid profiles in the bipotential cell differentiation of human promy-elocytic leukemia cell line HL-60, Blood 64 534-541. 

FIGURE 9.4 TLC-blotting/SIMS of various glycosphingolipids. (A) TLC profile of the separated glycosphingolipids, (B) MS profiles obtained by TLC-blotting/SIMS (a) spectrum of sulfatide peaks at m/z 862, 876, 890, 906, and 918 are molecular ions with different fatty acid species indicated in the figure (asterisks are peaks of triethanolamine as matrix) (b) spectrum of Gb Cer peaks at m/z 1309 and 1337 are molecular ions with different fatty acid species as indicated (c) spectrum of G peaks at m/z 1151, 1235, and 1263 are molecular ions with different fatty acid species as shown. Abbreviations LCB — long chain base, FA — fatty acid, hFA — hydroxyfatty acid. Hex — hexose, HexNAc — /V-acetylhexosamine. (From Taki, T. et al.. Anal. Biochem., 225, 24—27, 1995. With permission.) Continued. 

B. B. Reinhold, S.-Y. Chan, S. Chan, and V. N. Reinhold, Profiling glycosphingolipid structural detail Periodate oxidation, electrospray, collision-induced dissociation and tandem mass spectrometry, Org. Mass Spectrom., 29 (1994) 726-746. 

Souady, J., Soltwisch, J., Dreisewerd, K., Haier, J., Peter-Katalinic, J., and MUthing, J. 2009. Structural profiling of individual glycosphingolipids in a single thin-layer chromatogram by multiple sequential immunodetection matched with direct IR-M ALDI-o-TOF mass spectrometry. Anal. Chem., 81 9481-9492. 

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