Intestinal cell line, human fetal

Cell Lines. The human fetal intestinal cell line (FHS) was kindly supplied to us by Dr. Walter A. Nelson-Rees at the Naval Bioscience Laboratory, Oakland, California. The human colonic cell line, SKCO-1 developed by Drs. G. Trempe and L.F. Olds, was obtained from Dr. Jorgen Fogh, Sloan Kettering Institute, Rye, New York. The HT-29 cell line was developed and obtained from Dr. J. Fogh. SW-480 and SW-620 were developed at Scott and White Clinic in Temple, Texas and were obtained from Col. A. Liebovitz. 

Other cell lines used in permeability studies include the T84 human colonic adenocarcinoma colonic crypt cell model. This line has a reduced carrier expression, secrets mucus, and has very high resistance [31, 32], The IEC cell line is a rat fetal intestinal epithelium cell with higher permeabilities than Caco-2 cells [33], LLC PKi is a pig kidney epithelial cell line with low expression of efflux systems, but expression systems for transport proteins [32], 2/4/A1 cells are a conditionally immortalized rat fetal intestinal epithelium line with crypt cell-like morphology and temperature-sensitive differentiation [34], They form differentiated monolayers with tight junctions, increased brush border enzymes when grown on extracellular matrices with laminin. Transport of drugs with LP in 2/4/A1 monolayers was comparable to that in the human jejunum and up to 300 times faster than that in Caco-2 monolayers. In contrast, the permeability of HP drugs was comparable in both cell lines [34],... 

This study describes the effect of sodium butyrate on glyco-lipids from four human colonic tumor cell lines, SKCO-1, HT-29, SW-480 and SW-620 and a human fetal intestinal line, FHS. 

In the present study, we examined the pattern of fucolipids and gangliosides in cultured cell lines and alterations produced by a differentiating agent. A human fetal intestinal line (FHS), and four human colonic tumor lines (SKCO-1, HT-29, SW-480 and SW-620) were used. Cells were grown with or without sodium butyrate, (1.0 mM in SKCO-1 and 2.5 mM in all other cell lines) in... 

A1 ABC ATCC BCRP BSA Caco-2 cDNA CYP DMSO ECACC ER Conditionally immortalized cell line derived from fetal rat intestine ATP-binding cassette American-type culture collection Breast cancer-resistance protein (ABCG2) Bovine serum albumin Adenocarcinoma cell line derived from human colon Complementary DNA Cytochrome P450 Dimethyl sulfoxide European Collection of Cell Cultures Efflux ratio (transport basolateral-to-apical divided by transport a pical-to-basolateral)... 

Human / 4GalNAc-T3 is expressed in the intestines and testes, while / 4GalNAc-T4 is highly expressed in ovarian and fetal tissues and in a number of cancer cell lines./ 4GalNAc-T3 and T4 both act on GlcNAc/3-benzyl substrates as well as on core 2, 3, and 6 derivatives. 

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