Horseradish peroxidase biosensor

J.L. Tang, B.Q. Wang, Z.Y. Wu, X.J. Han, S.J. Dong, and E.K. Wang, Lipid membrane immobilized horseradish peroxidase biosensor for amperometric determination of hydrogen peroxide. Biosens. Bioelectron. 18, 867-872 (2003). 

Jia J, Wang B, Wu A, Cheng G, Li Z, Dong S. A method to construct a third-generation horseradish peroxidase biosensor self-assembling gold nanoparticles to three-dimensional sol-gel network. Anal Chem 2002 74 2217-... 

Wright, J.D., Rawson, K.M., Ho, W.O., Athey, D., and McNeil, C.J., 1995. Specific binding assay for biotin based on enzyme channelling with direct electron transfer electrochemical detection using horseradish peroxidase. Biosensors and Bioelectronics. 10 495-500. 

Yang SM, Li YM, Jiang XM, Chen ZC, Lin XF (2006) Horseradish peroxidase biosensor based on layer-by-layer technique for the determination of phenolic compounds. Sensor Actual B Chem 114(2) 774-780. doi 10.1016/j.snb.2005.07.035... 

Biosensors based on direct electron transfer of horseradish peroxidase... 

Horseradish peroxidase (HRP) is a member of the large class of peroxidases, which are enzymes defined as oxidoreductases using hydroperoxide as electron acceptor. HRP has been widely used for the construction of amperometric biosensor for the determination of H202 and small organic and inorganic substrates. 

B.H. Liu, F. Yan, J.L. Kong, and J.Q. Deng, A reagentless amperometric biosensor based on the coimmobilization of horseradish peroxidase and methylene green in a modified zeolite matrix. Anal. Chim. Acta 386, 31-39 (1999). 

A.L. Crumbliss, J.Z. Stonehuerner, R.W. Henkens, J. Zhao, and J.P. O Daly, A carrageenan hydrogel stabilized colloidal gold multi-enzyme biosensor electrode utilizing immobilized horseradish peroxidase and cholesterol oxidase/cholesterol esterase to detect cholesterol in serum and whole blood. Biosens. Bioelectron. 8, 331-337 (1993). 

T.J. Castilho, M. del P.T. Sotomayor, and L.T. Kubota, Amperometric biosensor based on horseradish peroxidase for biogenic amine determinations in biological samples. J. Pharmaceut. Biomed. Anal. 37, 785-791 (2005). 

Horseradish peroxidase (HRP) is an extracellular plant enzyme that acts in regulation of cell growth and differentiation, polymerization of cell wall components, and the oxidation of secondary metabolites essential for important pathogenic defense reactions. Because of these essential functions, and also because of its stability and ready availability, HRP has attracted considerable attention.13 It has been involved in a number of applications, such as diagnostic assays,14 biosensors,15 bioremediation,16 polymer synthesis,17 and other biotechnological processes.18 More applications in which HRP catalysis is translated into an electrochemical signal are likely to be developed in the near future. 

Bioelectrocatalysis involves the coupling of redox enzymes with electrochemical reactions [44]. Thus, oxidizing enzymes can be incorporated into redox systems applied in bioreactors, biosensors and biofuel cells. While biosensors and enzyme electrodes are not synthetic systems, they are, essentially, biocatalytic in nature (Scheme 3.5) and are therefore worthy of mention here. Oxidases are frequently used as the biological agent in biosensors, in combinations designed to detect specific target molecules. Enzyme electrodes are possibly one of the more common applications of oxidase biocatalysts. Enzymes such as glucose oxidase or cholesterol oxidase can be combined with a peroxidase such as horseradish peroxidase. 

Lu, X., Zhang, Q., Zhang, L., and Li, J., Direct electron transfer of horseradish peroxidase and its biosensor based on chitosan and room temperature ionic liquid, Electrochem. Commun., 8,874-878,2006. 

S. Razola Serradilla, B. Ruiz Lopez, N. Diez Mora, H.B. Mark and J.M. Kauffmann, Hydrogen peroxide sensitive amperometric biosensor based on horseradish peroxidase entrapped in a polypyrrole electrode, Biosens. Bioelectron., 17(11-12) (2002) 921-928. 

Index Entries Biosensors gasohol immobilized enzymes alcohol oxidase horseradish peroxidase. 

Acetylcholineesterase and choline oxidase A glassy C electrode surface was modified with osmium poly (vinyl-pyridine) redox polymer containing horseradish peroxidase (Os-gel-HRP) and then coated with a co-immobilized layer of AChE and ChO. A 22 pL pre-reactor, in which ChO and catalase were immobilized on beads in series, was used to remove choline. The variation in extracellular concentration of ACh released from rat hippocampal tissue culture by electrical stimulation was observed continuously with the online biosensor combined with a microcapillary sampling probe. Measurement of ACh and Ch was carried out by using a split disc C film dual electrode. 

Imabayashi S, Kong Y, Watanabe M (2001) Amperometric biosensor for polyphenol based on horseradish peroxidase immobilized on gold electrodes. Electroanalysis 13 408—412... 

Cui RJ, Huang HP, Yin ZZ, Gao D, Zhu JJ (2008) Horseradish peroxidase-functionalized gold nanoparticle label for amplified immunoanalysis based on gold nanoparticles/carbon nanotubes hybrids modified biosensor. Biosens Bioelectron 23 1666-1673... 

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