Enzymes channels

J. Zeravik, T. Ruzgas, and M. Franek, A highly sensitive flow-through amperometric immunosensor based on die peroxidase chip and enzyme-channeling principle. Biosens. Bioelectron. 18,1321—1327 (2003). 

Malaisse WJ, Zhang T-M, Verbruggen I, Willem R (1996) Enzyme to enzyme channeling of Krebs cycle metabolic intermediates in Caco-2 cells exposed to [2-13C]propionate. Biochem J 317 861-863... 

The original methods of fluorescence exciting transfer immunoassay (Ul) and enzyme channeling immunoassay (L9) are similar to the proximal linkage immunoassay. In the former, two reactants are coupled to solid antigen and antibody and, in the latter, they are coupled to two kinds of monoclonal antibodies. The enzyme enhancement immunoassay (G4) also falls in this category. 

L9. Litman, D. J., Hanlon, T. M., and Ullman, E. F., Enzyme channeling immunoassay A new homogeneous enzyme immunoassay technique. Anal. Biochem. 106, 223-229... 

The general principle of enzyme-channelling immunoassays, in which Ab-Ag binding brings two enzyme labels into close proximity to speed sequential reactions, is described in Fig. 8.4. One enzyme conjugate is... 

Fig. 8.4. Schematic illustration of the principle of the separation-free, amperometric enzyme channelling immunoassay with immobilized glucose oxidase and antibody on the PEI-modified electrode surface.

Triazines Flow-through amperometric immunosensor based on peroxidase chip and enzyme channeling system 1 ng L 1 8... 

True solution hybridization has been carried out with labeled probes using several approaches (i) selective destruction of unhybridized probes or their label (Section 7.3.1) (ii) strand displacement of a label probe (iii) energy transfer or enzyme channeling after two specific probes are brought together on the target. Particularly the first two approaches seem promising. 

I Gibbons, R Armenta, RK DiNello, et al. Nonseparation enzyme channeling immu-nometric assays. Meth Enzymol 136 93, 1987. 

Methylglutaryl-CoA hydratase (EC 4.2.1.18) catalyzes the reversible dehydration of HMG-CoA into 3-methylglutaconyl-CoA and is known from mammalian cells to be involved in leucine metabolism. This enzyme was detected in C. roseus suspension cultured cells and partially purified (87). Further enzymes channeling HMG-CoA away from isoprenoid biosynthesis in C. roseus cells were detected by a selective HPLC system showing that the CoA-esters may be rapidly dephosphorylated on the 3 -position by the action of 3 -nucleotidases (87). 

Miscellaneous Ho,E,C,l HRP/H2O2 Enzyme-channeling immunosensor 1000 cells mL- 93... 

Fig. 7 The principle of enzyme-channeling immunoassay using an electrode-bound enzyme to make the substrate for the enzyme label on the Ag. ...

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