Biogenic amines determination

T.J. Castilho, M. del P.T. Sotomayor, and L.T. Kubota, Amperometric biosensor based on horseradish peroxidase for biogenic amine determinations in biological samples. J. Pharmaceut. Biomed. Anal. 37, 785-791 (2005). 

According to some recent literature, typical conditions for biogenic amine determination are precolumn derivatization with Dnsl-Cl followed by separation on C8 or C18 column with gradient elution with mobile phases consisting of water or phosphate buffer and acetonitrile (or methanol) or postcolumn derivatization with OPA and gradient elution with mixtures of sodium acetate buffer and methanol (or acetonitrile). In the latter case, a counterion (such as hexanesulfonic or octansulfonic acid) is usually added in the mobile phase. 

Hernandez-Orte, P., Lapena, A. C., Pena-Gallego, A., Astrain, J., Baron, C., Pardo, 1., et al. (2008). Biogenic amine determination in wine fermented in oak barrels. Factors affecting formation. Food Research Intenuitioruil, 41, 697-706. http //dx.doi.org/ 10.1016/j.foodres.2008.05.002. 

Pena-GaUego, A., Hemandez-Orte, R, Cacho, J., Ferreira, V. (2009). Biogenic amine determination in wines nsing solid-phase extraction a comparative study. Journal of Chromatography A, 1216, 3398-3401. http //dx.doi.Org/10.1016/j.chroma.2009.01.106. 

Muresan, L Ronda Varela, R Frdbort, I. et al. (2008) Amine oxidase amperometric biosensor coupled to liquid chromatography for biogenic amines determination. Micro chim. Acta, 163, 219-225. 

Interlaboratory studies were carried out on the precision characteristics of the analytical methods used for determination of certain biogenic amines in fish and fish products, as required by German law. These included putrescine (4a), cadaverine (4b), tyramine (5) and histamine (6)78. 

Another application utilizing the coulometric array detector is the simultaneous determination of biogenic amines, kynurenine, and indole derivatives of tryptophan. The method employed a CIS column with a phosphate-acetate mobile phase (pH 4.1) containing methanol and sodium octyl sulphonate (Vaarman et al., 2002). 

Fluorescence detection, alone or with the aid of derivatizing reagents to enhance detector responses and improve the chromatographic resolution, has also been used for the determination of biogenic amines. Lakshmana and Trichur (1997) used native fluorescence to analyze NE, DA, and 5HT in rat brain utilizing an isocratic separation on an ODS CIS column. The detection limits reported were 100-250 pg on column. 

Chen Z, Wu J, Baker GB, Parent M, Dovichi NJ. 2001. Application of capillary electrophoresis with laser-induced fluorescence detection to the determination of biogenic amines and amino acids in brain microdialysate and homogenate samples. J Chromatogr A 914(1—2) 293-298. 

Durlu-Ozkaya, F., Alichanidis, E., Litopoulou-Tzanetaki, E. and Tunail, N. (1999). Determination of biogenic amine content of Beyaz cheese and biogenic amine... 

Kaniou, I., Samouris, G., Mouratidou, T., Eleftheriadou, A. and Zantopoulos, N. (2001). Determination of biogenic amines in fresh unpacked and vacuum-packed beef during storage at 4 degrees C, Food Chem., 74, 515. 

Pechanek, U., Pfannhauser, W. and Woidich, H. (1983). Determination of the content of biogenic amines in four food groups of Austrian marketplace, Z. Lebensmittel. Unters. Forsch., 176, 335. 

Shalaby, A.R. (1995). Multidetection, semiquantitative method for determining biogenic-amines in foods. Food Chem., 52, 367. 

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