High precision liquid chromatography HPLC

The concentration profiles of the reactant and the products were monitored by means of high precision liquid chromatography (HPLC), equipped with a Biorad Aminex HPX-87C carbohydrate column according to the method described in [4]. 

HPLC High-Precision Liquid Chromatography or High-Pressure Liquid Chromatography... 

HPLC-MS High-precision liquid chromatography None Mass spectrometry... 

Lanina et al. 1992 Oishi 1990). These methods include gas chromatography (GC) combined with mass spectrometry (MS) and high-performance liquid chromatography (HPLC) combined with an ultraviolet detector (UV). No comparisons can be made between methods since no data were given regarding sensitivity, recovery, or precision. 

High-performance liquid chromatography (HPLC), followed by GC/MS, has been used to fractionate and then quantitate the aliphatic and aromatic hydrocarbons present in liquid fuel precursors in order to determine the fuel potential of the compounds. Kerosene had the advantage of not requiring any sample preparation. Other light fuel oils may require the use of methylene chloride as a solvent prior to HPLC analysis (Lamey et al. 1991). The sensitivity, precision, and recovery of this method were not reported. 

Capable of the same quantitative accuracy and precision as high-pressure liquid chromatography (HPLC), particularly when used in conjunction with an internal... 

The combination of high-pressure liquid chromatography (HPLC) with monitoring by UV/visible detection provides an accurate, precise and robust method for quantitative analysis of pharmaceutical products and is the industry standard method for this purpose. 

When seeking a tool which could be used to determine the biological activity of mixture components in a more precise way, researchers directed their attention towards high-performance liquid chromatography (HPLC). Its advantage over TLC is its higher resolution, which helps to avoid false results caused by the co-elution of different compounds. 

The application of atomic spectroscopic instruments as element-specific detectors in chromatography has been reviewed by van Loon More recently, Krull has extensively reviewed their use in high pressure liquid chromatography (HPLC). Atomic spectrometry has found wide acceptance in the field of liquid chromatography because, in most cases, the fractions can be directly analysed after elution from the column. However, it is possible to use the technique for the analysis of solid samples without first dissolving the matrix. This is particularly useful after electrophoresis, where the fractions are fixed either in a gel or on paper. Kamel et al. have shown that it is possible to cut the appropriate sections and insert them into the carbon furnace for analysis. The disadvantage of this approach is that the precision is usually poorer (about 10%) and it is difficult to calibrate the instrument. Nevertheless, this approach is very useful if it is used for qualitative speciation. 

High-performance liquid chromatography (HPLC) is a fast technique that, with high precision and specificity, separates mixtures into individual ingredients. Used as a routine procedure, it has several advantages It can be completely automated sample cleanup and preparation are simple and the reproducibility of the packing material means that the analytical conditions remain the same for a new column. 

Compared to other bioanalytical methods such as high-performance liquid chromatography (HPLC), the methods used to quantitate mAbs often display less precision and a higher between-day variability. In choosing a bioanalytical method it must also be considered that some assays measure the unbound fraction, the bound fraction, or both. When using FACS, only the fraction of the therapeutic antibody that is bound to its antigen on the cells is counted. In contrast, ELISA measures only the unbound fraction in serum that can react with the offered antigen. 

In recent years, the availability of reproducible systems and precision instruments in high-performance liquid chromatography (HPLC) has prompted the application of chromatography in the rapid measurement of partition coefficients. In general, a linear relationship between log P and log K from a set of compounds is required for the interpolation or extrapolation of logP values of additional compounds of congeneric nature, as shown in Eq. (16) ... 

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