High performance liquid chromatography arsenic compounds

B.6 Speciation of Arsenic Compounds by Ion-Exchange High-Performance Liquid Chromatography with Hydride Generation Atomic Fluorescence Detection. 

Hansen, S.H., Larsen, E.H., Pritzi, G. and Cornett, C. (1992) Separation of seven arsenic compounds by high performance liquid chromatography with on-line detection by hydrogen-argon flame atomic absorption spectrometry and inductively coupled plasma mass spectrometry./. Anal. At. Spectrom., 1, 629-634. 

Saverwyns, S., Zhang, X.R., Vanhaecke, F., Cornelis, R., Moens, L. and Dams, R. (1997) Speciation of six arsenic compounds using high-performance liquid chromatography inductively coupled plasma mass spectrometry with sample introduction by thermospray nebulisation./. Chromatogr. A, 779, 299-306. 

Jyn Yynn and Wai [267] chelated organoarsenic compounds with sodium bis (trifluorethyl) dithiocarbamate prior to application of high performance liquid chromatography. He applied the technique to mixtures of arsenite, arsenate, dimethylarsonic acid, dimethylarsinic acid and other organoarsenic compounds. 

High performance liquid chromatography (Chapter 4) which is displacing conventional column chromatography (Chapter 6) has found numerous applications in the analysis of organic compounds, also metal organic compounds of mercury, tin, arsenic, manganese copper and lead. It has fewer applications in the determinations of anions and cations. 

U. Kohlmeyer, J. Kuballa, E. Jantzen, Simultaneous separation of 17 inorganic and organic arsenic compounds in marine biota by means of high-performance liquid chromatography/inductively coupled plasma mass spectrometry, Rapid Commun. Mass Spectrom., 16 (2002), 965D974. 

E. H. Larsen, G. Pritzl, S. H. Hansen, Speciation of eight arsenic compounds in human urine by high-performance liquid chromatography with inductively coupled plasma mass spectrometric detection using antimonate for internal chromatographic standardization, J. Anal. Atom. Spectrom., 8 (1993), 557-563. 

Coelho, N.M.M., CoeUio, L.M., de Lima, E.S., Pastor, A., de la Guardia, M. Determination of arsenic compounds in beverages by high-performance liquid chromatography-inductively coupled plasma mass spectrometry. Talanta 66, 818-822 (2005)... 

The concentrations of the three arsenicals (75-77) were determined in 37 marine organisms comprising algae, crustaceans, bivalves, fish and mammals by high-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICPMS) [170]. All three organoarsenics, which occurred at pg/kg concentrations, were detected in 25, 23 and 17 of the 37 samples analyzed, respectively. The limits of detection were 2-3 pg/kg dry mass. The data illustrate that all three compounds are common minor constituents in practically all marine samples. 

Zheng, B., Hinlelmann, H. Hyphenation of high performance liquid chromatography with sector field inductively coupled plasma mass spectrometry for the determination of ultra-trace level anionic and cationic arsenic compounds in freshwater fish. J Anal At Spectrom 2004,19, 191-195. Pergantis, S. A., Heithmar, E. M., Hinners, T. A. Speciation of arsenic animal feed additives by microbore high-performance liquid chromatography with inductively coupled plasma mass spectrometry. Analyst 1997, 122, 1063-1068. 

Wangkam, S., Pergantis, S. A. High-speed separation of arsenic compounds using narrow-bore high-performance liquid chromatography on-line with inductively coupled plasma mass spectrometry. J Anal At Spectrom 2000, 15, 627-633. 

W Goessler, A Rudorfer, EA Mackey, PR Becker, KJ Irgolic. Determination of arsenic compounds in marine mammals with high performance liquid chromatography and an inductively coupled plasma mass spectrometer as element specific detector. Appl Qrganomet Chem 12 491-501, 1998. 

Zheng.J., Goessler,W, and Kosmus,W. (1998). Speciation of arsenic compounds by coupling high-performance liquid chromatography with inductively coupled plasma mass spectrometry. Mikrochim.Acta 130(1-2), 71. 

Concentrations of total arsenic in soil and water samples contaminated with old Arsenical Munitions are not very useful to characterize the potential risks. Knowledge of which arsenic compounds are present in such samples is absolutely necessary to define toxicity. The identification of arsenic compounds requires a separation step combined with a detection step. For separation, gas chromatography and high performance liquid chromatography are widely used. Atomic absorption spectrometers, inductively coupled plasma optical emission spectrometers, and inductively coupled plasma mass spectrometers may serve as arsenic-specific detectors. 

Because the concentrations of arsenic compounds in soil and water samples are frequently low, only techniques of high sensitivity that enable accurate quantitative measurement to low concentration limits can be used for the determination of arsenic compounds. The hydride generation technique has low detection limits, but can be used only for the detection and quantification of arsenic compounds that are easily volatile or can be reduced to easily volatile arsines. High performance liquid chromatography will separate all arsenic compounds that can be detected by ICP-MS in the colunrn effluents. Arsenic compounds can still be quantified, when at least 0.1 ng of arsenic is present in the injected volume ( 100 mL). 

Chatterjee, A., Shibata, Y., Yoshinaga, J., and Morita, M. (2000) Detamination of arsenic compounds by high-performance liquid chromatography-ultrasonic nebulizer-high power... 

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