Guinea pig maximization test

Susten, A.S. and MJ. Breitenstein. 1990. Failure of acrolein to produce sensitization in the guinea pig maximization test. Contact Dermat. 22 299-300. 

Magnusson, B. and Kligman, A.M., The identification of contact allergens by animal assay. The guinea pig maximization test, J. Invest. Dermatol., 52, 268, 1969. 

Basketter, D.A. et al., Sulphanilic acid Divergent results in the guinea pig maximization test and the local lymph node assay, Contact. Derm., 27, 209, 1992. 

Basketter, D.A. and Scholes, E.W., Comparison of the local lymph node assay with the guinea-pig maximization test for detection of a range of contact allergens, Fd. Chem. Toxicol., 60, 65, 1992. 

Cell-mediated immunity Cytotoxic T lymphocyte killing delayed type hypersensitivity response mouse ear swelling test (MEST Gad et al., 1986) guinea pig maximization test (Magnusson and Kligman, 1969). 

FIGURE 15.5. Line chart for guinea pig maximization test for dermal sensitization. 

The guinea pig maximization test (GPMT) is a preferred method for the detection of skin sensitizers. It belongs to the class of adjuvant-tests, where the substance will be applied in Freund s complete adjuvant (FCA). The test is based on the possible induction of an immune response of the skin during an induction period (at least 1 week). This pretreatment of the subject will eventually result in a hypersensitive reaction during a further exposure, the so-called challenging phase. 

S. Frankild, A. Volund, J. E. Wahlberg, and K. E. Andersen. Comparison of the sensitivities of the Buehler test and teh guinea pig maximization test for predictive testing of contact allergey. Acta Derm. Venereol. 80 256-262 (2000). 

There are currently three OECD test guideline methods for test of skin sensitization in animals. These include the Guinea-Pig Maximization Test (GPMT), the Buehler test, and the murine Local Lymph Node Assay (LLNA). 

Bisphenol A causes slight skin and eye irritation. It did not cause contact allergy in a guinea pig maximization test. Furthermore, no cross-reactions were detected when animals sensitized to the diglycidyl ether of bisphenol A were tested with bisphenol A. 

Guinea Pig Maximization Test One of a number of skin tests for screening possible contact allergens. Considered to be a useful model for predicting likely moderate and strong sensitizers in humans. 

The skin permeability of this chemical is low the log Kp value from the reasoning algorithm is -6.856 and the chemical is known to give a weak response in the guinea pig maximization test, which indicates an equivocal response in mammals. This information leads to the prediction from the software that skin sensitization in humans is doubtful. 

The Guinea Pig Maximization Test combines FCA, irritancy, intradermal injection and occlusive topical... 

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