Green, Acid Methylene

Fig. 4a shows countercurrent chromatographic separation of four basic dyes, i.e., methyl green (MG), methylene blue (MB), neutral red (NR), and basic fuchsin using a two-phase solvent system composed of isoamyl alcohol-ethanol-acetic acid-distilled water (4 2 1 5, v/v). The first coil displays the separation of the mixture, and the other coils show distribution of individual dyes to demonstrate the reproducibility of the method. The separation was performed with 6 m of 0.35-mm-lD tubing (ca. 300 helical turns) at relative coil rotation of 0.25 rpm at 300 X g for 10 hr. 

Dinitro-l,5-dinydToxyanthraquinone-2,6-disu]fonic acid Methylene green from methylene blue. 

Hydrochloric acid, HCl, (1 1), 25 mL/S Mixed indicator. Methylene blue Methyl red, pH change 5.4 (base form - green acid form - lavender) pH indicator paper, wide range, 10 cm/S... 

Bromocresol Purple and Acid Blue 40, (9) Thymol Blue, (10) Acid Blue 113, (11) Fluorescein, (12) Methyl Green, (13) Methylene Blue and Acid Red 114, (14) Acridine Orange, (15) Nile Blue, (16) Rhodamine B, and (17) Malachite Green. 

Fig. 6-73. Separation of dyes on OmniPac PCX-500. - Eluant HCIO4 -NaCl04 - acetonitrile step-gradient flow rate 1 mL/min detection UV (254 nm) analytes (1) indigocarmine, (2) orange G, (3) tropaeolin O, (4) orange I, (5) alizarin red S, (5) orange II, (7) chromeazurol S, (8) bromocresol purple and acid blue 40, (9) thymol blue, (10) acid blue 113, (11) fluorescein, (12) methyl green, (13) methylene blue and acid red 114, (14) acridine orange, (15) nile blue, (16) rhodamine B, and (17) malachite green.

Ap4A, diadenosine tetraphosphate BBG, Brilliant blue green BzATP, 2 - 3 -0-(4-benzoyl-benzoyl)-ATP cAMP, cyclic AMP CCPA, chlorocyclopentyl adenosine CPA, cyclopentyl adenosine CTP, cytosine triphosphate DPCPX, 8-cyclopentyl-1,3-dipnopylxanthine IP3, inosine triphosphate lpsl, diinosine penta phosphate a,p-meATP, a,p-methylene ATP p.y-meATP, p.y-meihylene ATP 2-MeSADP, 2-methylthio ADP 2-MeSAMP, 2-methylthio AMP 2-MeSATP, 2-methylthio ATP NECA, 5 -W-ethylcarboxamido adenosine PPADS, pyridoxal-phosphate-6-azophenyl-2, 4 -disulfonic acid PLC, phospholipase C RB2, reactive blue 2 TNP-ATP, 2, 3 -0-(2,4,6-trinitrophenyl) ATP. 

The decolorization potential of immobilized P. chrysosporium MTCC 787 for azo dyes Acid Orange, Acid Red 114, triphenylmethane dye Methyl Violet, diazoic dye Congo Red, vat dye Vat Magenta, thiazine dye Methylene Blue, and anthraqui-none Acid Green was demonstrated by Radha et al. [53]. Decolorization experiments were carried out with immobilized calcium alginate (Ca-ALG) beads of different sizes (2-6 mm). 

Experiments—Pass hydrogen sulphide slowly into the most concentrated aqueous solution of Bindschedler s green obtainable, until after some time the colour has changed to yellowish-red. Now add dilute hydrochloric acid and the solution of 0-3 g. of sodium dichromate. Precipitate the methylene blue formed by adding zinc chloride solution. 

Nonchelating dyes include basic triphenylmethane dyes (e.g., Brilliant Green, Malachite Green, Crystal Violet), xanthene dyes (e.g., Rhodamine B, Rhodamine 6G), azine dyes (e.g., Methylene Blue), and acid dyes (e.g., Eosin, Erythrosin). These are intensely colored and when paired with an oppositely charged analyte ion lead to high sensitivities. 

Procedure. Steam out the distillation unit for 20 min. Pipette 5 ml of ammo-nium-N standard solution into the unit. Add 7 ml of sodium hydroxide solution and steam distil the liberated ammonia into 5 ml of boric acid solution. Collect 20 ml of distillate. Add 2-3 drops of methyl red-methylene blue solution and titrate with 0.01 M sulphuric acid until the green colour changes to... 

DMSO, Sodium azide, DINA, Methylene chloride. Sodium sulfate. Activated alumina Methyl green. Sodium picramate. Hydrochloric acid. Sodium nitrate... 

A few nitro derivatives of methylene blue have been described. By introducing one nitro group under mild conditions (nitric acid of ca. 20% and sodium nitrite) methylene green was obtained [85]. 

Zinc dust, hexachloroethane and aluminium Phosphorous pentoxide and phosphoric acid Sulfur, potassium nitrate and pitch Potassium chlorate, naphthalene and charcoal Zinc dust, hexachloroethane and naphthalene Silicon tetrachloride and ammonia vapour Auramine, potassium chlorate, baking soda and sulfur Auramine, lactose, potassium chlorate and chrysoidine Rhodamine red, potassium chlorate, antimony sulfide Rhodamine red, potassium chlorate, baking soda, sulfur Auramine, indigo, potassium chlorate and lactose Malachite green, potassium chlorate, antimony sulfide Indigo, potassium chlorate and lactose Methylene blue, potassium chlorate, antimony sulfide... 

Only aldehydes with an a-methylene group (i, e., R—CH2CHO) condense with 3,5-diaminobenzoic acid to form quinaldines.92 Consequently, in the carbohydrate series only 2-desoxysugars will react. The usual procedure adopted is to add to the unknown sugar solution an equal volume of a 1.3 percent solution of 3,5-diaminobenzoic acid hydrochloride in 50 percent aqueous perchloric acid. If the carbohydrate solution contains a 2-desoxysugar it will assume a yellow color with a green fluorescence.93 Use of an ester instead of a salt of 3,5-diaminobenzoic acid reduces the sensitivity of the test. 

Other drugs that change the color of the urine act as follows. Logwood (hematoxylon) does not color acid urine but produces a reddish or violet color in alkaline urine. Santonin imparts a yellowish color to acid urine, with a yellow foam if the urine is made alkaline, it imparts a very pronounced pink color. Picric acid gives reddish-brown color in both acid and alkaline urine. The various coal-tar products give a brownish-black color. Methylene blue imparts a green color. 

Extraction. One hundred grams of samples (corn, orange, potato, rice, cucumbers, lima beans, or green beans) were blended for five minutes with 300 ml of methylene chloride and 10 ml of 10% sulfuric acid. The filtered extract was dried over anhydrous sodium sulfate, and a 150 ml aliquot was concentrated on a Rinco evaporator to near dryness. The concentrate was dissolved into 10 ml of methylene chloride. 

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