Genotoxicity testing methods

Lohman PHM, Mendelsohn ML, Moore DH, et al. 1992. A method for comparing and combining shortterm genotoxicity test data The basic system. Mutat Res 266 7-25. 

Genotoxicity Test—ICH Mutation Cell Type Method... 

Brendler-Schwaab, S., Czich, A., Epe, B., Gocke, E., Kaina, B., Muller, L., PoDet, D. and Utesch, D. (2004) Photochemical genotoxicity principles and test methods. Report of a GUM task force. Mutation Research, 566, 65-91. 

Numerous test methods are available for assessing the genotoxic potential of substances. There are essentially three main categories of genotoxic damage that can be caused by a substance ... 

Ulitzur S. Bioluminescence test for genotoxic agents. Methods Enzymol. 1986 133 264-274. 

In this context, this chapter is meant to review the existing in vitro testing methods and update on the emerging in vitro approaches for the assessment of genotoxicity and carcinogenicity. 

Key words Mutagenicity, Genotoxicity, Carcinogenicity, In vitro assays, Test methods, Hazard identification... 

The micronucleus test in 3D human reconstructed skin models (RSMN) is one of the promising new test methods which aims at solving the low specificity issues shown by the standard in vitro genotoxicity tests and addressing the challenges presented by the requirements of the new Cosmetics Regulation [7],... 

Centre for the Validation of Alternative Methods (ECVAM) Scientific Advisory Committee (ESAC) on the scientific validity of the in vitro micronucleus test as an alternative to the in vitro chromosome aberration assay for genotoxicity testing. ESAC 25th meeting. http //ecvamjrcit/indexhtm. 

Speit, G., and Hartmann, A. (2005). The comet assay A sensitive genotoxicity test for the detection of DNA damage. Methods Mol Biol 291, 85-95. 

This book indudes 67 ASTM test methods, practices, and guides, covering field sampling, toxidty of water, sediment toxidty, fate related procedures, uses of data, avian test procedures, and genotoxicity tests. 

A group of about 10 participants discussed the "battery of acceptable tests to be used in the genotoxic evaluation of a new chemical/pesticide. Considerable concern was expressed about reliability and reproducibility of various test systems and reasons for these concerns were addressed. The test methods (assays) chosen should minimize variability. It was generally agreed that good predictors of carcinogenicity are currently available in the battery of mutagenicity assays. 

Genotoxicity. Potential genotoxicity of the new drug is evaluated using classical methods such as AMES test in bacteria and mammalian cells. The amount of drug used is typically much lower than in the standard test methods. 

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