3D human reconstructed skin models

In Vitro Micronucleus Test in 3D Human Reconstructed Skin Models (RSMN)... 

The micronucleus test in 3D human reconstructed skin models (RSMN) is one of the promising new test methods which aims at solving the low specificity issues shown by the standard in vitro genotoxicity tests and addressing the challenges presented by the requirements of the new Cosmetics Regulation [7],... 

Micronucleus assay 3D human reconstructed skin models Micronuclei induction Structural and numerical chromosome aberrations Genotoxicity hazard identification... 

Reus AA, Reisinger K, Downs TR, Carr G, Zeller A, Corvi R, Krul CAM Pfiihler S (2013) Comet assay in reconstructed 3D human epidermal skin models—investigation of intra- and inter-laboratory reproducibility with coded chemicals. Mutagenesis 28 709-720... 

Methods for in vitro reconstruction of three-dimensional (3D) differentiated human skin were developed during the 1980s (Bell et al., 1981). Cultured at the air-liquid interface (ALI), these in vitro reconstracted human epidermal (RhE) tissues display an in vivo-like stratified structure and functional epidermal layers including stratum comeum barrier. RhE may also be cocultured with dermal constructs containing viable fibroblasts (i.e., fuU-thickness models) as well as melanocytes (Fig. 12.1). The ALI culture format allows in vivo-like exposure and wounding scenarios including... 

---