Reporter gene fusions

In this work, we identified a c/.s-acting element associated with the hydA promoter of C. reinhardtii by introducing reporter gene fusions carrying various hydA 5 upstream regions into C. reinhardtii. Our results indicate that a sequence contained within the -128 to -21 region of the hydA promoter is sufficient to confer anaerobic specific gene expression. 

Kragelund, L., Christoffersen, B., Nybroe, O., and de Bruijn, F.J., Isolation of lux reporter gene fusions in Pseudomonas fluorescens DF57 inducible by nitrogen or phosphorus starvation, FEMS Microbiol. Ecol., 17, 95-106, 1995. 

Menne S, Walz M, Kiick U (1994). Expression studies with the bidirectional pcbAB-pcbC promotor region from Acremonium chrysogenum using reporter gene fusions. Appl. Microbiol. Biotechnol. 42 57-66. 

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