Luciferase gene fusions

Several fusions have been made at the 5 end of the luciferase gene without loss of enzymatic activity. The smallest fusion (in plasmid pKWlOl) is one which resulted in seven amino acids of the lambda cro protein being fused to a truncated luciferase missing the first six amino acids (24). An intermediate size fusion, resulted in twenty amino acids of cro protein fused onto the end of a truncated luciferase (pKWlOS)... 

Van Gelder You said that the luciferase knock-in behaviour is due to the presence of a single copy of wild-type Ter2 in this mouse. Does this fusion gene actually behave as a null if you put it over the Per2 ... 

Fig. 19. The Cx43 promoter region. Partial analysis of the human Cx43 gene extending from -360 to the site of fusion where De Leon et al. [1994] inserted the luciferase reporter gene at position +143. The transcription start site is numbered -1 [redrawn from De Leon et al., 1994]. The TATA box and the putative AP-1-binding sequence are underlined.

B. Induction of an NOS I-luciferase Fusion Gene by a Phorbol Ester... 

FIGURE 8 Expression of an NOSl -luciferase fusion gene in the central nervous system of a Drosophila embryo. A preblastoderm Drosophila embryo was microinjected with pNOS4.3-L and incubated at 17°C for 48 hr. X-Gal staining for P-galactosidase expression was performed on the first-instar larva as discussed in the text. 

Recent data demonstrate that the activities of certain transcription factors are regulated during the cell cycle (Dowdy etal., 1993). It is therefore possible that aberrant expression of NOSl-luciferase fusion genes in HeLa cells reflects the mitotic activity of the cell culture system. 

While transient expression of NOSl-luciferase fusion genes in HeLa cells remains enigmatic, CNS-specific expression of a NOSl-lacZ fusion... 

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