Reporter gene fusions genes

In this work, we identified a c/.s-acting element associated with the hydA promoter of C. reinhardtii by introducing reporter gene fusions carrying various hydA 5 upstream regions into C. reinhardtii. Our results indicate that a sequence contained within the -128 to -21 region of the hydA promoter is sufficient to confer anaerobic specific gene expression. 

Kragelund, L., Christoffersen, B., Nybroe, O., and de Bruijn, F.J., Isolation of lux reporter gene fusions in Pseudomonas fluorescens DF57 inducible by nitrogen or phosphorus starvation, FEMS Microbiol. Ecol., 17, 95-106, 1995. 

Menne S, Walz M, Kiick U (1994). Expression studies with the bidirectional pcbAB-pcbC promotor region from Acremonium chrysogenum using reporter gene fusions. Appl. Microbiol. Biotechnol. 42 57-66. 

Recent studies have further examined the iron stress response of pseudomonads using an iron-regulated, ice-nucleation gene reporter (inaZ) for induction of the iron stress response (17,18,84). This particular reporter system was developed by Loper and Lindow (85) for study of microbial iron stress on plant surfaces but was later employed in soil assays. In initial. studies, cells of Pseudomonas fluorescens and P. syringae that contained the pvd-inaZ fusion were shown to express iron-responsive ice-nucleation activity in the bean rhizosphere and phyllosphere. Addition of iron to leaves or soil reduced the apparent transcription of the pvd-inaZ reporter gene, as shown by a reduction in the number of ice nuclei produced. 

Figure 5.1. Yeast two-hybrid system. Interaction of proteins X and Y upstream of a reporter gene leads to transcriptional activation. Protein X is part of a fusion protein that binds to a site on DNA upstream of the reporter gene by means of a DNA binding domain. Protein Y is part of a fusion protein that contains a transcriptional activation domain. Interaction of proteins X and Y places the activation domain in the vicinity of the reporter gene and stimulates its transcription.

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