Protein gene fusion

Gene Fusion Systems for Isolation of Cloned Fusion Proteins ... 

Fraisier C, Abraham DA, van Oijen M, Cunliffe V, Irvine A, Craig R, Dzierzak EA (1998) Inhibition of Tat-mediated transactivation and HIV replication with Tat mutant and repressor domain fusion proteins. Gene Ther 5 946-954... 

Enright, A. J., Iliopoulos, I., Kyrpides, N. C., andOuzounis, C. A. (1999). Protein interaction maps for complete genomes based on gene fusion events. Nature 402, 86-90. 

Zhang, A., Gonzalez, S.M., Cantor, E.J., and Chong, S. (2001) Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins. Gene 275, 241-252. 

Figure 4. Leukaemic gene fusions involving HATs. (a) The t(8 16) translocation result in the MOZ-CBP fusion protein, while inv(8) gives rise to MOZ-T1F2. (b) Hypothetical model to explain how MOZ-T1F2 and MOZ-CBP fusions result in a similar leukaemia cell phenotype. See text for details, (c) The t(ll 16) and t(l 1 22) result in the MLL-CBP and MLL-p300 gene fusions, respectively...

Fused gene or gene pair analysis to estimate domain interaction Selection pressure promotes retention of gene fusion in ensuring protein domain interactions over the course of evolution. Symmetry and sequence clustering of gene sequences to detect fusion genes Functional associations of proteins [21,20]... 

FIGURE 5 Measuring [cAMP] with FRET. Gene fusion creates hybrid proteins that exhibit FRET when the PKA regulatory and catalytic subunits are associated (low [cAMP]). When [cAMP] rises, the subunits dissociate, and FRET ceases. The ratio of emission at 460 nm (dissociated) and 545 nm (complexed) thus offers a sensitive measure of [cAMP]. 

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