Food borne pathogens detection

Yu Y. X., Sun X. H., Pan Y. J., Zhao Y., 2010b. Research on Food-borne Pathogen Detection Based on Electronic Nose, chemistry online (in Chinese), 154-9. 

The piezoelectric transducer is suitable for DNA and protein detection with a detection limit of 1 ng cm (Nirschl et al., 2009). The piezoelectric sensor has been used in various applications such as cholera toxin diagnostic detection, hepatitis B, hepatitis C, and food-borne pathogen detection (Skladal et al., 2004 Serra et al., 2008 Chen et al., 2008). The piezoelectric transducer is a very sensitive method, noting that a detection limit of... 

Hill, W. E. The polymerase chain reaction Applications for the detection of food-borne pathogens. Crit. Rev. Food Sci. Nutr. 1996, 36,123-173. 

SPR biosensor-based detection of food contaminants, food-borne pathogens and toxins... 

Swaminathan, B., and Feng, P. (1994). Rapid detection of food-borne pathogenic bacteria. Annu. Rev. Microbiol. 48,401-426. 

Transcriptomic, proteomic, and/or metabolomic approaches have already been shown to be extremely useful to assess food safety and quality at every stage of production to ensure food safety for human consumption. They are also valuable tools to distinguish between similar food products and to detect food frauds (adulteration, origin, authenticity, etc.), food-borne pathogens, toxic species, food allergens, etc. 

Portable SERS Sensor for Sensitive Detection of Food-Borne Pathogens... 

Listeria spp. is probably one of the most seriously treated food-borne pathogens due to its ability to cause listeriosis, a condition with flu-like symptoms which can compromise the human immune system especially in pregnant women, children and the elderly. Mortality rates from full listeriosis are at 20% prompting the USDA and FDA to set a "zero tolerance" against the pathogen. Detection of this microbe is therefore more important than enumeration. In food. Listeria spp. can grow even at refrigerated temperatures and have been isolated from meat and cheese products. 

In our recent articles, we described the detection of a food-borne pathogen. Vibrio cholerae, which causes cholera disease. The assay relied on detection of Vibrio cholerae-specihc PCR amplicons using an electrochemical genosensor on screen-printed carbon chips. The signal was measured by intermittent pulse amperometiy (IPA) using a portable handheld reader AndCare (Alderon, Durham, NC). 

In the current chapter, a protocol is presented for top-down proteomic identification of protein biomarkers of food-borne bacterial microorganisms. The protein biomarkers were detected and analyzed using MALDI-TOF-TOF-MS/MS from bacterial cell lysates. Although the identification of food-borne pathogens is the practical objective of this work, this approach is applicable to other organisms. 

Food industry Biosensors based on CPs have been developed for quality control in food industry [113,225-227], The spectrum of these sensors ranges from detection of food-borne pathogens [228-234] and qualitative assessment of freshness of meat [235] to estimation of glucose, sucrose, lactate, citric acid, ascorbic acid, etc. in food products. 

Viswanathan, S., Rani, C. and Ho, J.A. (2012) Electrochemical immunosensor for multiplexed detection of food-borne pathogens using nanocrystal bioconjugates and MWCNT screen-printed electrode. Talanta, 94, 315-319. 

Low, K.-F., Chuenrangsikul, K., Rijiravanich, P. et al. (2012) Electrochemical genosensor for specific detection of the food-borne pathogen, vibrio cholerae. World J. Microbiol. BiotechnoL, 28 (4), 1699-1706. 

Arora, R, Sindhu, A., Dilbaghi, N., and Chaudhury, A. (2011) Biosensors as innovative tools for the detection of food borne pathogens. Biosens. Bioelectron., 28,1-12. Dweik, M., Stringer, R.C., Dastider, S.G., Wu, Y., Almasri, M., and Barizuddin, S. (2012) Specific and targeted detection of viable Escherichia coli 0157 H7 using a sensitive and reusable impedance biosensor with dose and time response studies. Taianta, 94, 84-89. 

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