Fluorescent imaging microscopy

Gadella, T. W. J. and Jovin, T. M. (1995). Oligomerization of epidermal growth-factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy—a stereochemical model for tyrosine kinase receptor activation. J. Cell Biol. 129, 1543-58. 

Electronic Absorption and Luminescence (Volume 12) Absorption and Luminescence Probes Fluorescence Imaging Microscopy Fluorescence Lifetime Measurements, Applications of Indirect Detection Methods in Capillary Electrophoresis Surface Measurements using Absorption/Luminescence... 

Luminescent RE + chelates have been successfully developed as labels and probes for highly sensitive and selective bioassays in the past two decades. Time-resolved Inmines-cence detection has been widely applied in fluoroimmunoassay, DNA hybridization assay, enzyme assay, cell activity assay and fluorescence imaging microscopy. ... 

Coley HM, Amos WB, Twentyman PR, Workman P. Examination by laser scanning confocal fluorescence imaging microscopy of the subcel-lular localisation of anthracyclines in parent and multidrug resistant cell lines. Br J Cancer 1993 67(6) 1316-23. 

Q. Li, T. Ruckstuhl, S. Seeger, Deep-UV laser-based fluorescence imaging microscopy of single molecules. J. Phys. Chem. B 108, 8324-8329 (2004)... 

Figure Bl.22.11. Near-field scanning optical microscopy fluorescence image of oxazine molecules dispersed on a PMMA film surface. Each protuberance in this three-dimensional plot corresponds to the detection of a single molecule, the different intensities of those features being due to different orientations of the molecules. Sub-diffraction resolution, in this case on the order of a fraction of a micron, can be achieved by the near-field scaiming arrangement. Spectroscopic characterization of each molecule is also possible. (Reprinted with pennission from [82]. Copyright 1996 American Chemical Society.)...

Clegg R M 1998 Fluoresoenoe resonanoe energy transfer Fluorescence Imaging Spectroscopy and Microscopy ed X F Wang and B Flerman, pp 179-251... 

Vol. 137. Fluorescence Imaging Spectroscopy and Microscopy. Edited by Xue Feng Wang and Brian Herman... 

Photothermal Spectroscopy Methods for Chemical Analysis. By Stephen E. Bialkowski Element Speclatlon in Bioinorganic Chemistry. Edited by Sergio Caroli Laser-Enhanced Ionization Spectrometry. Edited by John C. Travis and Gregory C. Turk Fluorescence Imaging Spectroscopy and Microscopy. Edited by Xue Feng Wang and Brian Herman... 

Clegg RM (1996) Fluorescence resonance energy transfer. In Wang XF, Flerman B (eds) Fluorescence imaging spectroscopy and microscopy. John Wiley, New York, pp 179-252... 

Tramier M, Coppey-Moisan M (2008) Fluorescence anisotropy imaging microscopy for homo-FRET in living cells. Methods Cell Biol 85 395-414... 

Clegg, R. M. (1996). Fluorescence resonance energy transfer. In Fluorescence Imaging. Spectroscopy and Microscopy. Vol. 137. (Wang, X. F. and Herman, B., eds.). John Wiley Sons, New York, pp. 179-252. 

Gadella, T., Jovin, T. and Clegg, R. (1994). Fluorescence lifetime imaging microscopy (FLIM) Spatial resolution of microstructures on the nanosecond time scale. Biophys. Chem. 48, 221-39. 

Holub, O., Seufferheld, M. J., Gohlke, C., Govindjee, G. J., Heiss, G. J. and Clegg, R. M. (2007). Fluorescence lifetime imaging microscopy of Chlamydomonas reinhardtii Non-photochemical quenching mutants and the effect of photosynthetic inhibitors on the slow chlorophyll fluorescence transients. J. Microsc. 226, 90-120. 

Marriott, G., Clegg, R. M., Arndt-Jovin, D. J. and Jovin, T. M. (1991). Time resolved imaging microscopy. Phosphorescence and delayed fluorescence imaging. Biophys. J. 60, 1374—87. 

While publications on fluorescence lifetime imaging microscopy (FLIM) have been relatively evenly divided between time and frequency domain methods, a majority of the 10 most highly cited papers using FLIM have taken advantage of the frequency domain method [1, 2-9]. Both techniques have confronted similar challenges as they have developed and, as such, common themes may be found in both approaches to FLIM. One of the most important criteria is to retrieve the maximum information out of a FLIM... 

Bastiaens, P. I. H. and Squire, A. (1999). Fluorescence lifetime imaging microscopy Spatial resolution of biochemical processes in the cell. Trends Cell Biol. 9, 48-52. 

Clayton, A. H. A., Hanley, Q. S. and Verveer, P. J. (2004). Graphical representation and multicomponent analysis of single-frequency fluorescence lifetime imaging microscopy data. J. Microsc. 213, 1-5. 

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