Fluorescence microscopy calcium imaging

The feasibility studies on single capsule detection of urea presence were carried out using confocal fluorescent microscopy. CLSM image of SNARF-1 dextran/urease capsules in absence and at O.IM urea added to the same capsules are presented on Figure 8. Small distinctions in the form and the sizes of capsule population are connected with non-uniformity of SNARF-1 dextran/urease CaCO particles received in co-precipitation process what is rather often observed for calcium carbonate templated capsules containing proteins [20]. 

Okano et al. [84] measured changes in cytoplasmic Ca2+ concentrations in platelets adhering to HEMA-STY block copolymer (HSB) surfaces by means of fluorescence microscopy combined with a high performance image processor. Comparative studies were also carried out with the HEMA-STY random (HSR) copolymer of poly HEMA and polystyrene. Their results showed that cytoplasmic free calcium levels in platelets that were in contact with the HEMA-STY... 

Similarly, live/dead images from fluorescence microscopy revealed viable cells on both scaffolds (not shown). However, the calcium content from each of the three hASC lines after 14 days of culture in osteogenic medium showed significantly higher calcium deposition on the collagen-PCL bicomponent scaffolds as compared to that on the pure PCL controls. On average,... 

Undesired effects in fluorescence confocal microscopy can be corrected by ratio imaging. Rati-oing (i.e., the compensation of two channels by digital division) can substantially reduce cross-talk effects in double detectors [63]. Environmental markers indicating the pH value or the level of free calcium ions (Ca"" ) also require ratio imaging to compensate for structural densities or section thickness [19]. 

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