Fluorescence microscopy image interpretation

The usehilness of FLIM is a result of the difficulties of quantifying fluorescence intensities in fluorescence microscopy. The steady-state fluorescence images can be difficult to interpret and quantify because there is no prac-... 

Fluorescence microscopy offers a number of advantages over other forms of microscopy (c/r. Tables 5.16 and 5.17). Its high sensitivity allows very low concentrations of specific substances to be localised. Because fiuorescence is observed as luminosity on a dark background, fluorescent constituents of the specimen can be seen even in extremely small amounts. Fluorescence microscopy can also be applied to detect particles below the resolution of a light microscope. Since fluorescence involves two wavelength bands (excitation and emission), optical specificity can substantially be increased. Fluorescence microscopy, because of its complexity, gives more difficulty than usual in interpretation of the image. 

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