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Total internal reflection fluorescence microscopy single-molecule imaging techniques

Abstract Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of biomolecules at tbe single-molecule level both in vitro and in living cells. This technique has opened a new era in bioscience, so-called single-molecule nanobioscience. In this review, we will summarize the theoretical basis of TIRFM, explain the instruments of TIRFM for single-molecules imaging, and discuss some novel applications of TIRFM to single-molecule nanobioscience. [Pg.77]


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Fluorescence images

Fluorescence imaging

Fluorescence imaging technique

Fluorescence imaging total internal

Fluorescence internal reflectance

Fluorescence microscopy

Fluorescence single-molecule

Fluorescence techniques

Fluorescent images

Fluorescent imaging

Fluorescent imaging microscopy

Fluorescent imaging total internal reflection fluorescence

Fluorescent technique

Image, 168 reflected

Imaging molecules

Imaging total internal reflection fluorescence

Internal fluorescence

Internal reflectance

Internal-reflection technique

Internally reflected

Microscopy fluorescent

Microscopy image

Microscopy imaging

Microscopy techniques

Molecule fluorescence

Molecule fluorescent

Reflectance technique

Reflectance total internal

Reflection imaging

Reflection technique

Reflectivity total

Single fluorescent molecules

Single image microscopy

Single molecule microscopy

Total internal reflectance fluorescence

Total internal reflectance fluorescence microscopy

Total internal reflection

Total internal reflection fluorescence

Total internal reflection fluorescence microscopy

Total internal reflection microscopy

Total internal reflection, fluorescent

Total reflection

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