Collagen fibronectin

The MMPs are a family of zinc-dependent neutral endopep-tidases that share structural domains but differ in substrate specificity, cellular sources, and inductivity (Table I). All the MMPs are important for remodeling of the extra cellular matrix and share the following functional features (/) they degrade extracellular matrix components, including fibronectin, collagen, elastin, proteoglycans, and laminin, (//) they are secreted in a latent proform and require activation for proteolytic activity, (///) they contain zinc at their active site and need calcium for stability, (/V) they function at neutral pH, and (v) they are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). 

Serum proteins from cell culture medium, fibronectin, collagen Native PS, pluronic F68, BSA... 

Fibronectin, collagen Photoreactive PVA modified with phenylazido groups Monkey kidney fibroblasts, murine macrophage-like cells, human liver carcinoma cells, mouse embryonic fibroblasts tCP of proteins onto hydrogel background and subsequent photoactivated immobilization 2005 [90]... 

First seeding fibronectin, collagen First seeding serum depletion of culture medium Coculture of rat hepatocytes and 3T3-J2 fibroblasts tFP of proteins 1998 [120]... 

Investigations of the underlying mechanism(s) revealed that the concentration, conformation, and bioactivity of vitronectin (a protein contained in serum that is known to mediate osteoblast adhesion ((Thomas et al., 1997) see the section Vitronectin ) was responsible for the select, enhanced adhesion (a crucial prerequisite for subsequent, anchorage-dependent-cell function) of osteoblasts on these novel nanoceramic formulations. Specifically, of the proteins (such as albumin, laminin, fibronectin, collagen, and vitronectin) tested, vitronectin adsorbed in the highest concentration on nanophase alumina after 4 hr moreover, competitive adsorption of vitronectin was 10% greater on nanophase compared to conventional alumina (Webster et al.,... 

Bare filter vs coated with fibronectin, collagen, endothelial cell monolayer, etc. and... 

Brennan, M. J., Oldberg, A., Hayman, E. G. and Ruoslahti, E. (1983). Effect of a proteoglycan produced by rat tumor cells on their adhesion to fibronectin-collagen substrata. Cancer Res. 43,4302-4307. 

Elice MJ, Urry LA, Hemler ME. Receptor frmcrions for the int rin VLA-3 fibronectin, collagen, and laminin binding are differentially influenced by Arg-Gly-Asp peptide and by divalent cations. J Cell Biol 1991 112 169-181. 

Integrins are the major type of cell surface receptors and are formed by a heterodimer with a and P subunits. Most integrins bind extracellular matrix components like fibronectin, collagen or intronectin. It seems that integrins activate PTKs clustering. 

TGF-B up-regulates Extracellular matrix proteins including fibronectins, collagens, laminin. 

TGF-P also regulates cell-substratum interactions by a selectively increasing expression of integrins, a major class of cell-adhesion receptors. Integrins bind fibronectin, collagens, and other ECM proteins28-29 and thus modulate the adhesion cascade, which modulates cell migration. 

Newgreen, D.F., Gibbins, I.L., Sauter, J., Wallenfels, B. and Wiltz, R. (1982) Ultrastructural and tissue eulture studies on the role of fibronectin, collagen and glycosaminoglycans in the migration of neural crest cells in the fowl embryo. Cell Tissue Res. 221 521-549. 

Integrins are the major cellular receptors for ECM proteins and provide a link between the internal cytoskeleton of cells (primarily the actin microfdament system) and extracellular proteins, such as fibronectin, collagen, and laminin. Integrins... 

Chitosan-collagen composite Isolated aortic valve endothelial cell (VEC) cultures and preferential adhesion to fibronectin, collagen types IV and I over laminin and osteo-pontin. Chitosan-collagen type IV films act as protein precoatings The composite showed improved VEC growth and morphology in comparison to chitosan alone Certain alteration in the properties of chitosan can improve amenability to valve tissue engineering applications Cuy et al. [126]... 

Integrins Transmembrane a)S-heterodimer a 165 kDa j , 90-120 kDa Plasmalemma Binding to laminins, fibronectin, collagen specificity depends on a)S composition Maintenance of cell to basement membrane and extracellular matrix binding... 

Fibronectin mediates the adhesion of cells to collagen by first binding to the collagen substrate followed by attachment of the cells to the fibronectin-collagen complex. Bovine brain gangliosides prevented the adhesion of cells to the fibronectin-collagen complex, but had no effect on the initial formation of the fibronectin-collagen complex. The order of efficiency was and G > Gd, j Gj > was not an inhibitor. Only the carbohydrate portions of... 

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