Ferguson analysis

Figure 4-26. Ferguson analysis for determining the molecular weights of native proteins. The mobilities of the unknown and standard proteins are measured in the same buffer conditions but at various acrylamide/ bis-acrylamide concentrations (CT) at least five different concentrations should be used, and the ratio of acrylamide bis-acrylamide should be held constant. From the slopes of the Ferguson plots (log Rf vs. CT) for the various proteins (unknown and standards) the molecular weights of the unknown can be determined.

P. Schneider, J. E. Ralton, M, J. McConville, and M. A. J. Ferguson, Analysis of the neutral glycan fractions of glycosyl-phosphatidylinositols by thin-layer chromatography. Ana/. Biochem. 210 106-112 (1993). 

Ferguson IF (1989) Auger microprobe analysis. Hilger, Bristol... 

Ferguson DM and Horwood LJ (2001). Cannabis use and traffic accidents in a birth cohort of young adults. Accident Analysis and Prevention, 33, 703-711. 

Ferguson PL, Iden CR, Brownawell BJ (2001) Analysis of nonylphenol and nonylphenol ethoxylates in environmental samples by mixed-mode high performance liquid chromatography-electrospray mass spectrometry. J Chromatogr A 938 79-91... 

Cotham WE, Metz TO, Ferguson PL, et al. Proteomic analysis of arginine adducts on glyoxal-modified ribonuclease. Mol. Cell. Proteomics 2004 3 1145-1153. 

D. M. Ferguson, G. L. Seibel, and P. A. Kollman, AMBER, a package of computer programs for applying molecular mechanics, normal mode analysis, molecular dynamics and free energy calculations to simulate the structural and energetic properties of molecules, Comp. Phys. Comm. 91 1 (1995). 

Doty, R.L. and Ferguson-Segall, M. (1989) Influence of adult castration on the olfactory sensitivity of the male rat a signal detection analysis. Behav. Neurosci. 103, 691-694. 

Ferguson et al. [18] reported on the application of a mixed-mode HPLC separation, coupled with ESI-MS for the comprehensive analysis of NPEOs and nonylphenol (NP) concentrations and distributions in sediment and sewage samples. The mixed-mode separation, which operates with both size-exclusion and reversed-phase mechanisms, allows the resolution of NPEO ethoxymers prior to introduction to... 

M [8]. Ferguson et al. [10] have shown that matrix effects may result in severe ion suppression in APEO analysis (this phenomenon occurs particularly in negative mode electrospray ionisation). They also demonstrated that the effect may be accounted for appropriately when using internal and surrogate standards, which closely mimic the ionisation behaviour of the analytes. In Ferguson s study, specially... 

A. Ferguson, T. C. Boles, C. P. Adams, and D. R. Walt. 1996. A Fiber Optic DNA Biosensor Microarray for the Analysis of Gene Expression. Nature Biotechnology 14 1681-1684. 

Ferguson P.L., C.R. Iden, and B.J. Brownawell (2000). Analysis of alkylophenol ethoxylate metabolites in the aquatic environment using liquid chromatography-electrospray mass spectrometry. Analytical Chemistry 72 4322-4330. 

If a product of template polymerization is composed of a daughter polymer and a template involved in polymer complex, the first step of analysis is separation of these two parts. Separation of two polymers forming a complex is sometimes difficult and depends on interactions between the components. Very often polymeric complexes are insoluble in water and also in organic solvents. In order to dissolve such compounds, aqueous or non-aqueous solutions of inorganic salts such as LiBr, LiCl, NH4CNS are used. Dimethylformamide or dimethylacetamide are commonly used as non-aqueous solvents. If one of the components is a polyacid, alkali solutions are used as solvent. Ferguson and Shah reported that the complex obtained by polymerization of acrylic acid in... 

A similar procedure was described by Eboatu and Ferguson. An object of analysis was the complex obtained by template polymerization of acrylic acid in the presence of poly(vinyl pyrrolidone). The polycomplex was dispersed in dry benzene and treated with diazomethane. The insoluble portion was filtered. The filtrate containing poly(methyl acrylate) was concentrated and finally dried. The insoluble fraction was scrubbed with methanol to extract polyCvinyl pyrrolidone). The residue was further washed with methanol and then dried. These three portions were characterized by IR spectroscopy. It was found that only about 70% separation of the complex is achieved. The occurrence of inseparable portion is attributed to a graft copolymer formation. For the separated... 

Of the various methods of weighted residuals, the collocation method and, in particular, the orthogonal collocation technique have proved to be quite effective in the solution of complex, nonlinear problems of the type typically encountered in chemical reactors. The basic procedure was used by Stewart and Villadsen (1969) for the prediction of multiple steady states in catalyst particles, by Ferguson and Finlayson (1970) for the study of the transient heat and mass transfer in a catalyst pellet, and by McGowin and Perlmutter (1971) for local stability analysis of a nonadiabatic tubular reactor with axial mixing. Finlayson (1971, 1972, 1974) showed the importance of the orthogonal collocation technique for packed bed reactors. 

The existence of a large number of bound electronic states, arising from 3P + 2P atomic limits, has now been determined statistically by Ferguson.81 This is now theoretically confirmed by our analysis of this system, which indicates 12 bound states of 02 , in addition to the 2Hg ground state, arising from the ground2/3 state of O" and the ground 3F and excited XD... 

Bushway, R.J., L.B. Perkins, L. Fukal, R.O. Harrison, and B.S. Ferguson (1991). Comparison of enzyme-linked immunosorbent assay and high-performance liquid chromatography for the analysis of atrazine in water from Czechoslavkia. Arch. Environ. Contam. Toxicol., 21 365-370. 

Hill WJ, Ferguson WS. 1979. Statistical analysis of epidemiological data from a chromium chemical manufacturing plant. J Occup Med 21 103-106. 

D. Ferguson, G. Seibel, and P. Kollman, Comput. Phys. Commun., 91,1 (1995). AMBER, a Package of Computer Programs for Applying Molecular Mechanics, Normal Mode Analysis, Molecular Dynamics and Free Energy Calculations to Simulate the Structural and Energetic Properties of Molecules. 

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