Modes of separation in HPLC

Discussed below are various modes of separations in HPLC. Included here is brief coverage of mobile-phase selection for various modes of chromatography and elementary information on mechanism, choice of solvents and columns, and other practical considerations. It should come as no surprise that reversed-phase HPLC is discussed at greater length in this section because it is the most commonly used technique in HPLC (more detailed discussion is provided in Section 15.8). Clearly,... 

It is clearly not possible to cover all these applications within the scope of this chapter. Detailed applications can be found in the literature [1-3,5,7]. Reproduced below is a case study to show how one may select a mode of separation in HPLC and at the same time develop a better understanding of separation mechanism. 

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