Fast atom bombardment mass spectroscopic

Gross, R. W. 1984. High plasmalogen and arachidonic acid content of canine myocardial sar-colemma A fast atom bombardment mass spectroscopic and gas chromatography-mass spectroscopic characterization, 23( 1) 158-65. 

Hazen, S. L., C. R. Hall, D. A. Ford, and R. W. Gross. 1993. Isolation of a human myocardial cytosolic phospholipase A2 isoform. Fast atom bombardment mass spectroscopic and reverse-phase high pressure liquid chromatography identification of choline and ethanolamine glycerophospholipid substrates. J CUnIme 91(6) 2513-22. 

A novel pigment has been isolated from the petals of Rosa hybrida with complex chromatographic techniques and the structure was elucidated with spectroscopic methods and high resolution fast-atom bombardement mass spectrometry, lH NMR, and FTIR. Anthocyanins were extracted from 7.9 kg of petals of Rosa hybrida cv. M me Violet with 80 per cent aqueous ACN containing 0.1 per cent TFA. The extract was purified in a Sephadex LH-20 column, and the fraction eluted with 80 per cent ACN was further fractionated in a HP-20 column using water, 15, 20 and 30 per cent ACN as mobile phases. The last fraction was lyophilized and separated by preparative RP-HPLC using an ODS column (50 X 5 cm i.d.). Solvents were 0.5 per cent aqueous TFA (A) and water-methanol... 

The structures of the novel ferrocenyl dendrimers 1-6 were straightforwardly established by a variety of spectroscopic and analytical techniques including H, C, and Si NMR and IR spectroscopies, fast atom bombardment mass spectrome-... 

The structures of 294-296 have been resolved unambiguously by fast atom bombardment mass spectrometry and 1FI-, 13C- and 19F- NMR spectroscopic studies316. 

References are given for spectroscopic studies, other v,c-oi IR hen these studies are reported in the synthetic/structural reference no further reference number is given. MS, mass spectrum FABMS, fast atom bombardment mass spectrum VT, variable-temperature. 

Mass spectrometry is a standard spectroscopic technique for the characterisation of high molecular weight organic and inorganic compounds, but has until recently received little attention from the zeolite community. The surface composition of zeolites has been explored using fast atom bombardment mass spectrometry (FABMS)[66] and secondary ion mass spectrometry [67], but mass spectrometric analysis of the bulk composition of a zeolite or of adsorbed molecules has not until very recently been attempted. The practical difficulty is to vaporise the solid. Two different strategies have been proposed laser ablation and plasma desorption. 

Ganong, B. R., Loomis, C. R., Hannun, Y. A., Bell, R. M. (1986). Specificity and mechanism of protein kinase C activation by sn-l,2-diacylglycerols. Proc. Natl. Acad. Sci. USA 83,1184-1188. Gross, R. W. (1984). High plasmalogen and arachidonic acid content of canine myocardial sarcolemma a fast atom bombardment mass spectroscc ic and gas chromatography-mass spectroscopic characterization. Biochemistry 23,158-165. 

HPLC methods previously described (22.24.26.42). The cinnamoyl ester-bearing compounds were detected by uitraviolet (UV) monitoring at 280 nm. Structure elucidation of the purified compounds was carried out by means of infrared (iR) and UV spectrophotometry, proton and carbon-13 nuciear magnetic resonance (NMR) spectroscopy, and electron impact (EI-) and fast-atom bombardment mass spectrometry (FAB-MS). The structures of the two new isolates and three of their derivatives were established on the basis of spectroscopic data and spectrai evidence obtained on comparison with azadirachtin (23). 

Physical Chemical Characterization. Thiamine, its derivatives, and its degradation products have been fully characterized by spectroscopic methods (9,10). The ultraviolet spectmm of thiamine shows pH-dependent maxima (11). H, and nuclear magnetic resonance spectra show protonation occurs at the 1-nitrogen, and not the 4-amino position (12—14). The H spectmm in D2O shows no resonance for the thiazole 2-hydrogen, as this is acidic and readily exchanged via formation of the thiazole yUd (13) an important intermediate in the biochemical functions of thiamine. Recent work has revised the piC values for the two ionization reactions to 4.8 and 18 respectively (9,10,15). The mass spectmm of thiamine hydrochloride shows no molecular ion under standard electron impact ionization conditions, but fast atom bombardment and chemical ionization allow observation of both an intense peak for the patent cation and its major fragmentation ion, the pyrimidinylmethyl cation (16). 

Mass spectroscopy is a useful technique for the characterization of dendrimers because it can be used to determine relative molar mass. Also, from the fragmentation pattern, the details of the monomer assembly in the branches can be confirmed. A variety of mass spectroscopic techniques have been used for this, including electron impact, fast atom bombardment and matrix-assisted laser desorption ionization (MALDI) mass spectroscopy. 

We report on the further physical and chemical characterization of the new forms of molecular carbon, C ) and C70. Our results demonstrate a high yield of production (14%) under optimized conditions and reveal only and C70 in measurable quantity, in an 8S IS ratio. These two new molecular forms of carbon can be completely separated in analytical amounts by column chromatography on alumina. Comparison among mass spectra obtained by the electron impact, laser desorption, and fast atom bombardment (FAB) methods allows a clear assessment of the composition of the mixed and pure samples, and of the fragmentation and double ionization patterns of the molecules. In addition, spectroscopic analyses are reported for the crude mixture by C NMR and by IR spectroscopy in KBr pellet, and for pure C o and C70 in solution by UV-vis spectroscopy. 

This paper describes the further physical and chemical characterization of these two new forms of molecular carbon." Our results include the high-yield production (14%) of soluble material under optimized conditions, consisting of only C o and C70 in measurable quantity. These have been separated in analytical amounts by column chromatography and have been characterized in pure or mixed forms by a combination of electron impact, fast atom bombardment (FAB), and laser desorption mass spectrometry. Spectroscopic characterization is reported including the C NMR spectrum and the infrared absorption spectrum for the crude... 

Mass spectrometry (MS) in its different detection modes is now the ultimate method for the characterization of the mass, composition, and sequence of peptide or protein components in a mixture and is particularly well suited to the mobile phases employed in RPC. Electrospray ionization (ESI) and matrix-assisted laser desorption ionization time-of-fiight (MALDl-TOF) mass spectroscopic methods, and to a lesser extent thermospray and fast atom bombardment... 

Mass spectroscopy has been reviewed, particularly its application to volatiles [1,2] and non-volatiles [3], fast atom bombardment techniques [4] (Section 2.6), secondary ion mass spectroscopic techniques (SIMS) [5] (Section 2.1), electrospray ionisation (ESI) - mass spectroscopy (Section 2.3) [6] and laser mass spectroscopy techniques (Section 2.2) [7]. 

About ten years ago the author speculated that between the extremes mentioned above a third set of reaction conditions and a third group of reaction products might exist, namely reaction conditions yielding soluble multicyclic polymers. Such polycondensations yield a complex mixture of reaction products which cannot satisfactorily be characterized by classical spectroscopic methods such as IR-, NMR and NMR spectroscopy. Mass spectrometric methods such as Fast-Atom-Bombardment, and above all, MALDI-TOF mass spectrometry are required, and even these methods do not answer all questions concerning the structure of the reaction products. Hence, a systematic exploration of this new working field was not feasible before the year 2000, i. e. before powerful MALDI-TOF mass spectrometer became commercially available. 

---