Expression of recombinant

Mayerhofer, R., Langridge, W. H. R., Cormier, M. J., and Szalay, A. A. (1995). Expression of recombinant Renilla luciferase in transgenic plants results in high levels of light emission. Plant. J. 7 1031-1038. [Pg.418]

Jebanathirajah, J. A. Andersen, S. Blagoev, B. Roepstorff, P. A rapid screening method to monitor expression of recombinant proteins from various prokaryotic and eukaryotic expression systems using matrix-assisted laser desorption ioniza-tion-time-of-flight mass spectrometry. Anal. Biochem. 2002, 305, 242-250. [Pg.151]

A final problem for bioinformatics and bioanalytical scientists is the characterization of engineered microorganisms. Whole-cell analysis by mass spectrometry has been used to confirm the introduction of therapeutic genes into adenovirus vectors,100 to confirm the expression of recombinant proteins in bacteria,101,102 and also in vaccinology.103 In the broader case, identification of... [Pg.269]

Sorensen, H.P and Mortensen, K.K. (2005) Soluble expression of recombinant proteins in the cytoplasm of Pichia pastoris. Microbial Cell Factories, 4 (1), 1. [Pg.53]

Yazdani, S. and Mukherjee, K. (2004) Continuous-culture studies on the stability and expression of recombinant streptokinase in Escherichia coli. Bioprocess and Biosystems Engineering, 24, 341-346. [Pg.282]

Venegas, A., Goldstein, J.C., Beauregard, K, Oles, A., Abdulhayoglu, N. and Fuhrman, J.A. (1996) Expression of recombinant microfilarial chitinase and analysis of domain function. Molecular and, Biochemical Parasitology 78, 149-159. [Pg.218]

Volume 306. Expression of Recombinant Genes in Eukaryotic Systems Edited by Joseph C. Glorioso and Martin C. Schmidt... [Pg.30]

Hayhurst A., Happe S., Mabry R., Koch Z., Iverson B.L., Georgiou G., Isolation and expression of recombinant antibody fragments to the biological warfare pathogen Brucella melitensis, J. Immunol. Meth. 2003 276 185-196. [Pg.453]

The expression of recombinant proteins in cells in which they do not naturally occur is termed heterologous protein production (Chapter 3). The first biopharmaceutical produced by genetic engineering to gain marketing approval (in 1982) was recombinant human insulin (tradename Humulin ), produced in E. coli. An example of a more recently approved biopharmaceutical that is produced in E. coli is that of Kepivance, a recombinant keratinocyte growth factor used to treat oral mucositis (Chapter 10). Many additional examples are provided in subsequent chapters. [Pg.106]

Expression of recombinant proteins in animal cell culture systems... [Pg.109]

Starting Down the Path to Rituxan High-Level Expression of Recombinant Proteins... [Pg.570]

Bartelsen, O., Lansmann, S., Nettersheim, M., Lemm, T., Ferlinz, K., and Sandhoff, K., 1998, Expression of recombinant human acid sphingomyeUnase in insect Sf21 cells purification, processing and enzymatic characterization. J Biotechnol 63 29-40. [Pg.302]

Better, M.D., Improved methods and bacterial cells for expression of recombinant protein products. PCTInt. Appl, 2001, WO 2001073082. [Pg.114]

Cloning and Expression of Recombinant Myoglobin Active Site Variants of Myoglobin... [Pg.1]

The successful expression of recombinant plant peroxidases such as HRP C has been a major focus of research in a number of laboratories. Three synthetic HRP C genes based on the amino acid sequence determined by Welinder (36, 47) were synthesized independently in order to initiate this work (63-65). A number of different expression systems have been evaluated (64, 66-73), a summary of which is presented in Table I. Refolding of recombinant HRP C isolated from inclusion... [Pg.112]

Knaust, R. K. and Nordlund, R (2001). Screeningfor soluble expression of recombinant proteins in a 96-weU format. Anal. Biochem. 297,79-85. [Pg.42]

The expression of recombinant proteins in cells in which they do not naturally occur is termed heterologous protein production . By far the most common microbial species used to produce... [Pg.112]

The progress of reverse chemical genetics research is influenced by the efficiency of generation of active recombinant proteins. In recent years, numerous systems for the expression of the recombinant proteins have been developed. Of these, the baculovirus system is considered to be the most efficient. Typically in the baculovirus system, an insect cell line (for example, Sf9) is used as a host for the expression of recombinant proteins. In the present report, we describe the novel application of Kaiko as a host in the baculovirus system for the expression of recombinant... [Pg.109]

Jones D, Kroos N, Anima R, van Montfort B, Vooys A, van der Kraats S, van der Helm E, Smits S, Schouten J, Brouwer K, Lagerwerf F, van Berkel P, Opstelten DJ, Logtenberg T, Bout A. High-level expression of recombinant IgG in the human cell line Per.C6. Biotechnol Prog 2003 19 163-168. [Pg.158]

Expression of Recombinant Antibody Fusion Proteins in E. coli... [Pg.419]

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