Recombinant in plants

This food reserve is not, however, a limited one that we steadily deplete. Our store of carbohydrates—and the oxygen to go with it—is constantly replenished by the recombining, in plants, of carbon dioxide and water. The energy for recombination comes, of course, from the sun. 

Boyko, A., Filkowski, J., Hudson, D., Kovalchuk, 1. 2006a. Homologous recombination in plants is organ specific. Mutat Res 595(1-2) 145-155. 

Puchta, H. 2002. Gene replacement by homologous recombination in plants. Trends Plant Sci 1 340-348. 

Wright, D.A. et al. (2005) High-frequency homologous recombination in plants mediated by zinc-finger nucleases. Plant J. 44, 693-705... 

In recent years, plants have emerged as a promising new system for the production of recombinant proteins. Plants are widely described as green factories because they provide possible solutions to several of the safety and economic concerns raised by animal systems. The advantages of plants include economical large-scale... 

Several soluble antigens that have been produced in plants using plant viruses as expression systems have shown immunogenic and protective properties in test animals [11]. The plant-derived proteins, which include known immunogens from pathogens or allergens from plants [38], have been tested either as crude plant extracts containing recombinant protein or as purified material from the infected plants. 

Within each species, individual promoters resulted in distinct, tissue-dependent accumulation patterns. The cauliflower mosaic virus (CaMV) 35S promoter, for example, led to high-level accumulation in callus and leaves whereas the maize ubiqui-tin-1 promoter was the best choice for producing recombinant proteins in cereal seeds even though it is not in itself seed-specific [23]. The lack of such comparative studies for proteins other than rAbs makes it difficult to generalize an optimal expression strategy for all proteins. Tables 7.1 and 7.2 list recombinant proteins expressed in plants and provide details of the production system, promoters and other regulatory elements used in each case. 

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