Designer genes

Hayashi C.Y. and Lewis R.V., Spider flagelUfotm silk Lessons in protein design, gene structure, and molecular evolution, BioEssays, 23, 750, 2001. 

Recombinant DNA technology can also be used to design genes that encode for proteins with desired features [34]. The gene can be incorporated into a plasmid, which is then used to transform a bacterial host such as Escherichia coli. Finally, the production of the desired amino acid polymer is performed by the host with a precisely defined sequence and near absolute monodispersity [29, 35]. 

Pioneering studies have demonstrated the potential of gene therapy for the treatment of inherited hematopoietic diseases [440300], and particularly for ADA-SCID [470017], [470024], [666662], [666664], [666665]. However, vector design, gene transfer protocols and inadequate engraftment and expansion of genetically engineered cells limited the success of earlier studies [206054], [657269], [657273], [668669]. 

Vinson, C. R., Hai, J. W., and Boyd, S. M. (1993). Dimerization specificity of the leucine zipper-containing BZIP motif in DNA binding — Prediction and rational design. Genes Dev. 7, 1047-1058. 

Italics are used to designate genes, whereas mRNAs and proteins are nonitali-cized by convention. 

Complex Gene designation Gene product Amino acid residues... 

Designer Genes for Field Use Monsanto Seeking Permit to Test Revolutionary Pesticide", Sacramento Bee, December 23, 1984. 

Baker, A. H. (2004) Designing gene delivery vectors for cardiovascular gene therapy. Prog Biophys Mol Biol 84 279-299. 

Hasty, J. Isaacs, R Dolnik, M. McMUlen, D. Collins, J. J. Designer gene networks towards fundamental cellular control. Chaos 2001, II, 207-220. 

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